Panasenko O M, Shalina R I, Azizova O A
Biull Eksp Biol Med. 1985 Apr;99(4):434-7.
Spin probes were used to study alteration of red cell membranes in nephropathy of varying degree of gravity. Iminoxyl radicals of the lipid nature were applied as spin probes, in particular 2-(3-carboxypropyl)-4,4-dimethyl-2-tridecyl-3-oxazolidinyl (probe I). It was shown that in nephropathy, the orderliness parameter increases and the hydrophoby of probe I localized in a red cell suspension of nephropathy patients diminishes as compared with analogous parameters in healthy pregnant women. This attests to both immobilization of the fatty acid chains of phospholipids and to an increase in the polarity of the lipid bilayer in the area of probe localization. It was established that diminution of probe I hydrophoby is in a satisfactory agreement with the disease gravity and the degree of edema in patients. It was noted that alterations discovered in red cell membranes in nephropathy are similar to those seen during activation of lipid peroxidation in membranes. The possibility of lipid peroxidation involvement into the pathogenesis of nephropathy is discussed.
使用自旋探针研究不同严重程度肾病中红细胞膜的变化。将具有脂质性质的亚胺氧基自由基用作自旋探针，特别是2-(3-羧丙基)-4,4-二甲基-2-十三烷基-3-恶唑烷基（探针I）。结果表明，在肾病中，与健康孕妇的类似参数相比，肾病患者红细胞悬液中探针I的有序参数增加，疏水性降低。这证明了磷脂脂肪酸链的固定以及探针定位区域脂质双层极性的增加。已确定探针I疏水性的降低与患者的疾病严重程度和水肿程度令人满意地一致。值得注意的是，肾病中红细胞膜发现的变化与膜脂质过氧化激活时所见的变化相似。讨论了脂质过氧化参与肾病发病机制的可能性。
