a Institute of Cotton Research, Chinese Academy of Agricultural Science, State Key Laboratory of Cotton Biology, Anyang 455000, China.
b College of Life Sciences, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Genome. 2018 Jul;61(7):539-547. doi: 10.1139/gen-2017-0237.
WD40 repeat proteins are largely distributed across the plant kingdom and play an important role in diverse biological activities. In this work, we performed genome-wide identification, characterization, and expression level analysis of WD40 genes in cotton. A total of 579, 318, and 313 WD40 genes were found in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. Based on phylogenetic tree analyses, WD40 genes were divided into 11 groups with high similarities in exon/intron features and protein domains within the group. Expression analysis of WD40 genes showed differential expression at different stages of cotton fiber development (0 and 8 DPA) and cotton stem. A number of miRNAs were identified to target WD40 genes that are significantly involved in cotton fiber development during the initiation and elongation stages. These include miR156, miR160, miR162, miR164, miR166, miR167, miR169, miR171, miR172, miR393, miR396, miR398, miR2950, and miR7505. The findings provide a stronger indication of WD40 gene function and their involvement in the regulation of cotton fiber development during the initiation and elongation stages.
WD40 重复蛋白广泛分布于植物界，在多种生物活性中发挥着重要作用。在这项工作中，我们对棉花中的 WD40 基因进行了全基因组鉴定、特征描述和表达水平分析。在陆地棉、亚洲棉和雷蒙德氏棉中分别发现了 579、318 和 313 个 WD40 基因。基于系统发育树分析，WD40 基因分为 11 组，组内具有高度相似的exon/intron 特征和蛋白结构域。WD40 基因的表达分析表明，它们在棉花纤维发育的不同阶段（0 和 8 DPA）和棉花茎中存在差异表达。鉴定出了一些 miRNA 靶向 WD40 基因，这些基因在棉花纤维起始和伸长阶段的发育中起着重要作用。其中包括 miR156、miR160、miR162、miR164、miR166、miR167、miR169、miR171、miR172、miR393、miR396、miR398、miR2950 和 miR7505。这些发现为 WD40 基因的功能及其在棉花纤维起始和伸长阶段发育调控中的作用提供了更强的证据。
