在非洲爪蟾卵母细胞中注入的DNA上组装的两种不同类型的微型染色体的结构。

Structure of the two distinct types of minichromosomes that are assembled on DNA injected in Xenopus oocytes.

作者信息

Ryoji M, Worcel A

出版信息

Cell. 1985 Apr;40(4):923-32. doi: 10.1016/0092-8674(85)90352-6.

Abstract

DNA injected into germinal vesicles of Xenopus oocytes is assembled into two distinct types of minichromosomes. One type is soluble and behaves like conventional nucleosomal chromatin. The other type is insoluble, is sensitive to DNAase I and to micrococcal nuclease, lacks a canonical nucleosome repeat, and generates a half-nucleosome size limit digest with micrococcal nuclease. We suggest that these peculiar minichromosomes may be the ones that display the unconstrained, "dynamic" DNA supercoils in the living oocyte.

摘要

注入非洲爪蟾卵母细胞生发泡的DNA组装成两种不同类型的微型染色体。一种类型是可溶的，其行为类似于传统的核小体染色质。另一种类型是不溶的，对DNA酶I和微球菌核酸酶敏感，缺乏典型的核小体重复序列，并且用微球菌核酸酶产生半核小体大小限制的消化产物。我们认为，这些特殊的微型染色体可能是在活卵母细胞中显示无约束的“动态”DNA超螺旋的那些染色体。