Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
Chair and Department of Biology and Genetics, Medical University of Lublin, Chodźki 4a, 20-094 Lublin, Poland.
Talanta. 2018 Sep 1;187:120-124. doi: 10.1016/j.talanta.2018.05.024. Epub 2018 May 8.
The conditions of micellar electrokinetic capillary chromatography for separation and simultaneous measurement of coumarins (coumarin, scoparone, isoscopoletin, esculin, esculetin, umbelliferone) including furanocoumarins (xanthotoxin, byakangelicin, isopimpinellin, bergapten, phellopterin, xanthotoxol) have been elaborated. The influence of different parameters, such as the pH of the buffer, sodium cholate (SC) or methanol concentration in the buffer, on the migration time, peak resolution, peak asymmetry, and number of theoretical plates was investigated. The optimum separation of the compounds was achieved using 50-µm i.d. capillaries with a total length of 64.5 cm (56 cm effective length) and a buffer system at pH 9.00 consisting of 50 mM sodium tetraborate, 45 mM SC, and 20% of methanol (v/v). The developed method ensured good repeatability of corrected peak areas and migration times (the relative standard deviations were in the range of 2.8-6.1% and 0.8-4.0%, respectively). The average limit of detection for all studied compounds was below 1.3 µg mL. Moreover, good linearity of the relationship between the peak corrected area and the concentration of the compounds was observed (correlation coefficient >0.99). The method was successfully applied in the quantitative analysis of two different types of samples, i.e. Heracleum sphondylium herb and Aesculus hippocastanum cortex.
已经详细阐述了胶束电动毛细管色谱法分离和同时测定香豆素（香豆素、scoparone、isoscopoletin、esculetin、esculetin、umbelliferone）包括呋喃香豆素（xanthotoxin、byakangelicin、isopimpinellin、bergapten、phellopterin、xanthotoxol）的条件。研究了不同参数（例如缓冲液的 pH 值、胆酸钠 (SC) 或缓冲液中甲醇浓度）对迁移时间、峰分辨率、峰不对称性和理论塔板数的影响。使用内径为 50-µm、总长度为 64.5 cm（有效长度为 56 cm）的毛细管和 pH 9.00 的缓冲液系统（由 50 mM 四硼酸钠、45 mM SC 和 20%甲醇（v/v）组成）实现了化合物的最佳分离。所开发的方法确保了校正峰面积和迁移时间的良好重复性（相对标准偏差分别在 2.8-6.1%和 0.8-4.0%范围内）。所有研究化合物的平均检测限均低于 1.3 µg mL。此外，观察到峰校正面积与化合物浓度之间的关系具有良好的线性（相关系数>0.99）。该方法成功应用于两种不同类型样品的定量分析，即鄂西獐牙菜全草和欧洲七叶树皮质。
