Department of Botany, Mycology and Ecology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Int J Mol Sci. 2021 Apr 30;22(9):4756. doi: 10.3390/ijms22094756.
Due to global warming, invasive species have spread across the world. We therefore studied the impact of short-term (1 day or 2 days) and longer (7 days) heat stress on photosynthesis and secondary metabolites in , one of the important invasive species in the European Union. leaves exposed to short-term heat stress (35 °C/1 d) showed a decrease in chlorophyll and maximum potential quantum efficiency of photosystem II (Fv/Fm) compared to control, 35 °C/2 d, or 30 °C/7 d treatments. In turn, the high level of lipid peroxidation and increased HO accumulation indicated that the 30 °C/7 d stress induced oxidative damage. The contents of xanthotoxin and bergapten were elevated in the 2 d and 7 d treatments, while isopimpinellin was detected only in the heat-stressed plants. Additionally, the levels of free proline and anthocyanins significantly increased in response to high temperature, with a substantially higher increase in the 7 d (30 °C) treatment. The results indicate that the accumulation of proline, anthocyanins, and furanocoumarins, but not of phenolic acids or flavonols, contributes to protection of plants against heat stress. Further studies could focus on the suppression of these metabolites to suppress the spread of this invasive species.
由于全球变暖，入侵物种已经遍布世界各地。因此，我们研究了短期（1 天或 2 天）和长期（7 天）热应激对光合作用和次生代谢物的影响，这是欧盟重要的入侵物种之一。与对照（35°C/1d、35°C/2d 或 30°C/7d）相比，短期热应激（35°C/1d）处理的 叶片的叶绿素和光系统 II 的最大潜在量子效率（Fv/Fm）下降。反过来，高水平的脂质过氧化和增加的 HO 积累表明 30°C/7d 应激诱导了氧化损伤。在 2d 和 7d 处理中，黄樟素和补骨脂素的含量升高，而异茴芹素仅在受热胁迫的植物中检测到。此外，游离脯氨酸和花青素的含量在高温下显著增加，7d（30°C）处理的增加幅度明显更高。结果表明，脯氨酸、花青素和呋喃香豆素的积累有助于 植物抵御热应激，但不包括酚酸或类黄酮。进一步的研究可以集中于抑制这些代谢物以抑制这种入侵物种的传播。
