Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Talanta. 2018 Sep 1;187:179-187. doi: 10.1016/j.talanta.2018.05.033. Epub 2018 May 9.
An electrochemiluminescence (ECL) biosensor based on functional electrospun nanofibers for hybridization detection of specific CdkN2A/p16 anti-oncogene at trace level via binding luminescent composite nanoparticles for signal amplification has been developed. The carboxylated multiwalled carbon nanotubes (MWCNTs) doped polycaprolactam 6 (PA6) electrospun nanofibers (PA6-MWCNTs) was prepared via electrospinning, which served as the nanosized backbones for silica nanoparticles (SiO) electrodeposition. The functional electrospun nanofibers (PA6-MWCNTs-SiO) used as supporting scaffolds for single-stranded DNA1 (ssDNA1) immobilization can dramatically increase the amount of DNA attachment and the sensitivity of hybridization. The sandwich construction of ssDNA1-CdkN2A/p16 anti-oncogene -tri(2,2'-bipyridyl)ruthenium(II) (Ru(bpy))/silver nanoparticles (AgNPs) doped gold (Au) core-shell luminescent composite nanoparticles (RuAg@AuNPs)-labeled ssDNA2 (RuAg@Au-ssDNA2) was fabricated through a hybridization reaction. It was observed that high amount of doped Ru(bpy) in RuAg@AuNPs successfully amplify the recognition signal by adding tripropylamine (TPrA). The change of ECL intensity was found to have a linear relationship in respect to the logarithm of the CdkN2A/p16 anti-oncogene concentrations in the wide range of 1.0 × 10~1.0 × 10 M, with a detection limit of 0.5 fM (S/N = 3) which is comparable or better than that in reported anti-oncogene assays. Excellent sensitivity and selectivity make the developed biosensor a promising tool for the detection of tumor biomarkers.
基于功能化静电纺纳米纤维的电化学发光（ECL）生物传感器，通过结合发光复合纳米粒子进行信号放大，用于痕量水平特异性 CdkN2A/p16 抗癌基因的杂交检测。通过静电纺丝制备了掺杂羧基多壁碳纳米管（MWCNTs）的聚己内酯 6（PA6）电纺纳米纤维（PA6-MWCNTs），用作硅纳米粒子（SiO）电沉积的纳米级骨架。作为单链 DNA1（ssDNA1）固定化的支撑支架，功能化电纺纳米纤维（PA6-MWCNTs-SiO）可以显著增加 DNA 附着量和杂交的灵敏度。通过杂交反应制备了 ssDNA1-CdkN2A/p16 抗癌基因-三（2,2'-联吡啶）钌（II）（Ru(bpy))/银纳米粒子（AgNPs）掺杂金（Au）核壳发光复合纳米粒子（RuAg@AuNPs）-标记的 ssDNA2（RuAg@Au-ssDNA2）的三明治结构。观察到 RuAg@AuNPs 中掺杂的 Ru(bpy) 量高，通过添加三丙胺（TPrA）成功放大了识别信号。发现 ECL 强度的变化与 CdkN2A/p16 抗癌基因浓度的对数在 1.0×10~1.0×10 M 的宽范围内呈线性关系，检测限为 0.5 fM（S/N=3），与已报道的抗癌基因检测相当或更好。出色的灵敏度和选择性使开发的生物传感器成为检测肿瘤生物标志物的有前途的工具。
