Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Guangdong Provincial Key Laboratory of Proteomics and Key Laboratory of Transcriptomics and Proteomics of Ministry of Education of China, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.
Department of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba, Manitoba Institute of Child Health, Winnipeg, MB R3T 2N2, Canada.
Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:159-167. doi: 10.1016/j.msec.2018.04.045. Epub 2018 Apr 17.
Bone marrow-derived mesenchymal stem cells (BMSCs) are easy to collect and culture, and it is identified that it has multi-directional differentiation potential, moreover it has low immunogenicity, hence it can be used as an allogeneic cell source for skin wound healing. Hydrogel has been widely used in skin wound healing own to it is able to mimic the 3D microenvironment of cells, which supports cell proliferation, migration and secretion. In this study, we created a novel biocompatible thermo-sensitive hydrogel to carry BMSCs for full-thickness skin wound healing. The thermo-sensitive hydrogel loaded with BMSCs can fast achieve sol-gel transition after implanting to the wound. Histological results confirmed that hydrogel-BMSCs combination group showed significant promotion of wound closure, epithelial cells' proliferation and re-epithelialization, and reduced inflammatory responses in the wounds and in the tissues surrounding the wounds. The combination therapy also can promote collagen deposition, TGF-β1 and bFGF secretion and tissue remodeling. The present study provides a promising strategy for the clinical treatment of skin wounds.
骨髓间充质干细胞（BMSCs）易于采集和培养，且被鉴定具有多向分化潜能，同时免疫原性低，因此可作为皮肤伤口愈合的同种异体细胞来源。水凝胶因其能够模拟细胞的 3D 微环境而被广泛应用于皮肤伤口愈合，支持细胞增殖、迁移和分泌。本研究中，我们构建了一种新型的生物相容性温敏水凝胶，以携带 BMSCs 促进全层皮肤伤口愈合。负载 BMSCs 的温敏水凝胶在植入伤口后能迅速实现溶胶-凝胶转变。组织学结果证实，水凝胶-BMSCs 联合组显著促进了伤口闭合、上皮细胞增殖和再上皮化，并减少了伤口及周围组织中的炎症反应。联合治疗还能促进胶原沉积、TGF-β1 和 bFGF 分泌及组织重塑。本研究为皮肤伤口的临床治疗提供了一种有前景的策略。
