Gao Chang, Du Yue, Wang Xin, Cao Huikun, Lin Bin, Liu Youping, Di Xin
School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
Bioorg Med Chem Lett. 2018 Jul 15;28(13):2265-2269. doi: 10.1016/j.bmcl.2018.05.039. Epub 2018 May 18.
As part of a bioprospecting program aimed at the discovery of anti-inflammatory agents from the Corydalis bungeana Turcz. (C. bungeana), five new hexahydrobenzophenanthridine alkaloids, corycaline A-E (1-5), along with four known alkaloids, were isolated from the whole plant of C. bungeana. Their structures including absolute configurations were elucidated on the basis of extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. The inhibitory activities of the nine compounds on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 mouse macrophage cells were determined; all tested compounds except 2 and 7 exhibited significant inhibitory effects with IC values in the range of 1.00-2.79 μM.
作为一项旨在从地锦草中发现抗炎剂的生物勘探计划的一部分，从地锦草全株中分离出了五种新的六氢苯并菲啶生物碱，即紫堇碱A - E（1 - 5）以及四种已知生物碱。基于广泛的光谱分析和电子圆二色性（ECD）计算，阐明了它们的结构，包括绝对构型。测定了这九种化合物对RAW 264.7小鼠巨噬细胞中脂多糖（LPS）诱导的一氧化氮（NO）产生的抑制活性；除2和7外，所有测试化合物均表现出显著的抑制作用，IC值在1.00 - 2.79μM范围内。
