Kadan Sleman, Sasson Yoel, Saad Bashar, Zaid Hilal
Qasemi Research Center, Al-Qasemi Academic College, P.O. Box 124, 30100 Baqa Al-Gharbiyye, Israel.
Casali Center for Applied Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Givat Ram, 91904 Jerusalem, Israel.
Evid Based Complement Alternat Med. 2018 Apr 26;2018:8294320. doi: 10.1155/2018/8294320. eCollection 2018.
In the present study, we tested the chemical composition, cytotoxicity and antidiabetic activity of two distinct extracts of wild Artichoke-like vegetable, : methanol and hexane. GC/MS phytochemical analysis of methanol and hexane extracts revealed 39 compounds reported here for the first time in out of the 45 detected compounds. Only Stigmasterol was present in both extracts. The efficacy of extracts in enhancing glucose transporter 4 (GLUT4) translocation to the plasma membrane (PM) was tested in L6 muscle cells stably expressing myc-tagged GLUT4 (L6-GLUT4myc) using cell-ELISA test. Results obtained here indicate that methanol and hexane extracts were safe up to 250 g/ml as measured with MTT and the LDH leakage assays. The methanol extract was the most efficient in GLUT4 translocation enhancement. It increased GLUT4 translocation at 63 g/ml 1.5- and 2-fold relative to the control in the absence and presence of insulin, respectively. These findings indicate that possesses antidiabetic activity in part by enhancing GLUT4 translocation to the PM in skeletal muscle.
在本研究中，我们测试了野生洋蓟样蔬菜的两种不同提取物（甲醇提取物和己烷提取物）的化学成分、细胞毒性和抗糖尿病活性。对甲醇提取物和己烷提取物进行的气相色谱/质谱联用（GC/MS）植物化学分析显示，在检测到的45种化合物中，有39种化合物在此首次报道。两种提取物中均仅存在豆甾醇。使用细胞酶联免疫吸附测定法（cell-ELISA test），在稳定表达myc标签的葡萄糖转运蛋白4（GLUT4）的L6肌肉细胞（L6-GLUT4myc）中测试了提取物增强葡萄糖转运蛋白4（GLUT4）向质膜（PM）转位的功效。此处获得的结果表明，通过MTT和乳酸脱氢酶（LDH）泄漏测定法测得，甲醇提取物和己烷提取物在高达250μg/ml时是安全的。甲醇提取物在增强GLUT4转位方面最为有效。在不存在和存在胰岛素的情况下，它在63μg/ml时分别使GLUT4转位相对于对照增加了1.5倍和2倍。这些发现表明，该植物部分通过增强骨骼肌中GLUT4向质膜的转位而具有抗糖尿病活性。
