光诱导 T-jump 和时间分辨 X 射线溶液散射揭示的胰岛素六聚体解离动力学。
Insulin hexamer dissociation dynamics revealed by photoinduced T-jumps and time-resolved X-ray solution scattering.
机构信息
Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
出版信息
Photochem Photobiol Sci. 2018 Jul 11;17(7):874-882. doi: 10.1039/c8pp00034d.
Abstract
The structural dynamics of insulin hexamer dissociation were studied by the photoinduced temperature jump technique and monitored by time-resolved X-ray scattering. The process of hexamer dissociation was found to involve several transient intermediates, including an expanded hexamer and an unstable tetramer. Our findings provide insights into the mechanisms of protien-protein association.
摘要
采用光致温度跃变技术研究了胰岛素六聚体解离的结构动力学，并通过时间分辨 X 射线散射进行了监测。研究发现，六聚体解离过程涉及几个瞬态中间体，包括扩展的六聚体和不稳定的四聚体。我们的研究结果为蛋白质-蛋白质缔合机制提供了深入的了解。
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