Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, MD, 20892-0520, USA.
Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, MD, 20892-0520, USA.
Curr Opin Struct Biol. 2021 Oct;70:99-107. doi: 10.1016/j.sbi.2021.05.002. Epub 2021 Jun 25.
Time-resolved small- and wide-angle X-ray scattering studies of proteins in solution based on the pump-probe approach unveil structural information from intermediates over a broad range of length and time scales. In spite of the promise of this methodology, only a fraction of the wealth of information encoded in scattering data has been extracted in studies performed thus far. Here, we discuss the methodology, summarize results from recent time-resolved X-ray scattering studies, and examine the potential to extract additional information from these scattering curves.
基于泵浦-探测方法的溶液中蛋白质的时间分辨小角和广角 X 射线散射研究揭示了广泛长度和时间尺度范围内的中间体的结构信息。尽管该方法具有很大的前景，但迄今为止的研究中仅提取了散射数据中编码的信息的一小部分。在这里，我们讨论了该方法，总结了最近的时间分辨 X 射线散射研究的结果，并研究了从这些散射曲线中提取更多信息的潜力。
