Mitsumori Makoto
Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan.
Methods Mol Biol. 2018;1796:57-65. doi: 10.1007/978-1-4939-7877-9_5.
To isolate strictly anaerobic rumen bacteria capable of degrading cellulose, environmental and nutritional conditions similar to the rumen environment should be simulated in vitro. One of the most useful techniques for isolating rumen bacteria is the roll-tube technique. In this chapter, the roll-tube technique for isolating cellulolytic rumen bacteria is briefly outlined.
为了分离出能够降解纤维素的严格厌氧瘤胃细菌，应在体外模拟与瘤胃环境相似的环境和营养条件。分离瘤胃细菌最有用的技术之一是滚管技术。在本章中，将简要概述用于分离纤维素分解瘤胃细菌的滚管技术。
