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细胞群体密度和血清对3T3细胞(钠钾)-ATP酶的影响。

The influence of cell population density and serum on the (Na+K)-ATPase of 3T3 cells.

作者信息

McCaldin B, Tenang E M

出版信息

J Cell Physiol. 1985 Jun;123(3):449-50. doi: 10.1002/jcp.1041230323.

DOI:10.1002/jcp.1041230323
PMID:2985633
Abstract

The (Na+K)-ATPase of 3T3 cells has been measured as a function of culture density and the type of serum in which the cells were grown. When 3T3 cells were grown in medium containing 10% newborn calf serum their (Na+K)-ATPase activity increased as the culture density increased and the cells became quiescent at densities of about 17.5 X 10(4) cells/cm2. When these cells are subcultured into medium containing 10% foetal bovine serum the activity of the enzyme decreases as the culture density increases and the cells attain final densities of about 5 X 10(4) cells/cm2.

摘要

已对3T3细胞的(钠钾)-ATP酶进行了测量,该酶是培养密度以及细胞生长所用血清类型的函数。当3T3细胞在含有10%新生牛血清的培养基中生长时,其(钠钾)-ATP酶活性随着培养密度的增加而升高,并且细胞在密度约为17.5×10⁴个细胞/平方厘米时进入静止期。当将这些细胞传代培养到含有10%胎牛血清的培养基中时,该酶的活性随着培养密度的增加而降低,并且细胞达到约5×10⁴个细胞/平方厘米的最终密度。

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