School of Physics and Electronic Engineering, Xinyang Normal University, Xinyang 464000, China.
Key Laboratory of Microelectronics and Energy of Henan Province, Xinyang Normal University, Xinyang 464000, China.
Sensors (Basel). 2018 Jun 1;18(6):1765. doi: 10.3390/s18061765.
Cardiac biomarkers (CBs) are substances that appear in the blood when the heart is damaged or stressed. Measurements of the level of CBs can be used in course of diagnostics or monitoring the state of the health of group risk persons. A multi-region bio-analytical system (MRBAS) based on magnetoimpedance (MI) changes was proposed for ultrasensitive simultaneous detection of CBs myoglobin (Mb) and C-reactive protein (CRP). The microfluidic device was designed and developed using standard microfabrication techniques for their usage in different regions, which were pre-modified with specific antibody for specified detection. Mb and CRP antigens labels attached to commercial Dynabeads with selected concentrations were trapped in different detection regions. The MI response of the triple sensitive element was carefully evaluated in initial state and in the presence of biomarkers. The results showed that the MI-based bio-sensing system had high selectivity and sensitivity for detection of CBs. Compared with the control region, ultrasensitive detections of CRP and Mb were accomplished with the detection limits of 1.0 pg/mL and 0.1 pg/mL, respectively. The linear detection range contained low concentration detection area and high concentration detection area, which were 1 pg/mL⁻10 ng/mL, 10⁻100 ng/mL for CRP, and 0.1 pg/mL⁻1 ng/mL, 1 n/mL⁻80 ng/mL for Mb. The measurement technique presented here provides a new methodology for multi-target biomolecules rapid testing.
心脏生物标志物 (CBs) 是心脏受损或受到压力时出现在血液中的物质。CBs 水平的测量可用于诊断过程或监测高危人群的健康状况。提出了一种基于磁阻抗 (MI) 变化的多区域生物分析系统 (MRBAS)，用于超灵敏同时检测 CBs 肌红蛋白 (Mb) 和 C 反应蛋白 (CRP)。该微流控装置使用标准微加工技术设计和开发，可在不同区域使用，这些区域已预先用特定的抗体进行修饰，以进行特定的检测。Mb 和 CRP 抗原标签与选定浓度的商业 Dynabeads 连接，然后被捕获在不同的检测区域中。在初始状态和存在生物标志物的情况下，对三重敏感元件的 MI 响应进行了仔细评估。结果表明，基于 MI 的生物传感系统对 CBs 的检测具有高选择性和灵敏度。与对照区域相比，CRP 和 Mb 的超灵敏检测的检测限分别为 1.0 pg/mL 和 0.1 pg/mL。线性检测范围包含低浓度检测区和高浓度检测区，CRP 的检测范围为 1 pg/mL-10 ng/mL，10-100 ng/mL，Mb 的检测范围为 0.1 pg/mL-1 ng/mL，1 n/mL-80 ng/mL。这里提出的测量技术为多靶标生物分子的快速检测提供了一种新方法。
