Laboratorio de Fisiopatología Cardiovascular, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Facultad de Ciencias Médicas, CONICET-Universidad Nacional de Cuyo, M5502JMA Mendoza, Argentina.
Laboratorio de Bioquímica Vegetal, Instituto de Biología Agrícola de Mendoza (IBAM), Facultad de Ciencias Agrarias, CONICET-Universidad Nacional de Cuyo, M5528AHB Chacras de Coria, Argentina.
Arch Biochem Biophys. 2018 Aug 1;651:28-33. doi: 10.1016/j.abb.2018.05.021. Epub 2018 May 31.
Grape pomace extract (GPE) is a rich and relatively low-cost source of phenolic compounds. However, little is known about the main GPE metabolites in mammals, which could help explain the observed health-promoting effects. This study investigated the presence of parent compounds from flavanol, flavonol and stilbene families and their metabolites in rat plasma and tissues after an acute intake of GPE in doses of 300 and 600 mg kg/body weight. The measurement of free compounds and their metabolites was performed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Results showed the presence of epicatechin, epicatechin methyl-glucuronide, epicatechin methyl-sulphate, catechin, catechin-glucuronide, quercetin methyl-glucuronide, resveratrol-3-glucuronide, resveratrol-4-glucuronide and resveratrol-3-sulphate in plasma, which was dose dependent. The most abundant measured compound in plasma was epicatechin-glucuronide. The presence of glucuronidated and methyl-glucuronidated forms of catechin were observed in the liver at both doses, while epicatechin-glucuronide and methyl-glucuronide were detected only upon intake of 600 mg GPE/kg body weight. At this dose epicatechin-glucuronide and methyl-glucuronide were also detected in muscle, and catechin methyl-glucuronide in adipose tissue. Results show the main GPE metabolites present in rat tissues after oral consumption, contributing to better understand the health benefits of GPE and its potential utilization as a functional ingredient.
葡萄渣提取物 (GPE) 是一种富含酚类化合物且相对廉价的来源。然而，人们对哺乳动物中 GPE 的主要代谢物知之甚少，这些代谢物有助于解释观察到的促进健康的作用。本研究在大鼠急性摄入 300 和 600mg/kg 体重剂量的 GPE 后，调查了类黄酮、黄酮醇和芪类化合物的母体化合物及其代谢物在血浆和组织中的存在情况。通过超高效液相色谱-串联质谱法 (UHPLC-MS/MS) 测量游离化合物及其代谢物。结果表明，在血浆中存在表儿茶素、表儿茶素甲基葡萄糖苷、表儿茶素甲基硫酸盐、儿茶素、儿茶素葡萄糖苷、槲皮素甲基葡萄糖苷、白藜芦醇-3-葡萄糖苷、白藜芦醇-4-葡萄糖苷和白藜芦醇-3-硫酸盐，且呈剂量依赖性。血浆中含量最丰富的测定化合物是表儿茶素葡萄糖苷。在肝脏中，两种剂量下均检测到儿茶素的葡萄糖醛酸和甲基葡萄糖醛酸形式，而仅在摄入 600mg GPE/kg 体重时才检测到表儿茶素葡萄糖苷和甲基葡萄糖苷。在该剂量下，表儿茶素葡萄糖苷和甲基葡萄糖苷也在肌肉中检测到，而儿茶素甲基葡萄糖苷在脂肪组织中检测到。结果表明，口服 GPE 后大鼠组织中存在主要的 GPE 代谢物，有助于更好地理解 GPE 的健康益处及其作为功能性成分的潜在利用。
