Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN-CSIC) , José Antonio Nováis, 10 , 28040 Madrid , Spain.
Facultad de Veterinaria , Universidad Complutense , 28040 Madrid , Spain.
J Agric Food Chem. 2019 Apr 24;67(16):4463-4471. doi: 10.1021/acs.jafc.9b00222. Epub 2019 Apr 12.
Grape byproducts are rich sources of polyphenols with powerful antioxidant and health-promoting effects. The impact of supplementing chicken diets with grape byproducts on plasma and thigh meat concentrations of phenolic metabolites was evaluated by analyzing samples by high-performance liquid chromatography quadrupole time of flight mass spectrometry. Chickens were fed three experimental diets: Control diet, Control+8% grape pomace, and Control+0.1% grape seed extract. In plasma, 32 phenolic metabolites were identified, some of which were conjugated catechin/epicatechin metabolites exclusively identified in chickens fed diets enriched in grape byproducts. Also, these chickens showed significantly higher plasmatic concentrations of 21 phenolic metabolites. In thigh meat, 14 phenolic metabolites were identified, but no differences were found between diets. Higher plasmatic tocopherol was found when supplementing diets with grape byproducts, while no changes were observed in meat. Thus, supplementing chicken diets with grape byproducts leads to a significant increase in the circulation of phenolic metabolites and tocopherol.
葡萄副产物是多酚的丰富来源，具有强大的抗氧化和促进健康的作用。通过高效液相色谱四极杆飞行时间质谱分析样品，评估了在鸡饲料中添加葡萄副产物对血浆和大腿肉中酚类代谢物浓度的影响。鸡喂食三种实验性日粮：对照日粮、对照+8%葡萄渣和对照+0.1%葡萄籽提取物。在血浆中，鉴定出 32 种酚类代谢物，其中一些是仅在喂食富含葡萄副产物日粮的鸡中鉴定出的共轭儿茶素/表儿茶素代谢物。此外，这些鸡的 21 种酚类代谢物的血浆浓度明显更高。在大腿肉中，鉴定出 14 种酚类代谢物，但日粮之间没有差异。添加葡萄副产物会使血浆中叶醌素的含量升高，而肌肉中的含量没有变化。因此，在鸡饲料中添加葡萄副产物会导致酚类代谢物和生育酚的循环显著增加。
