Shen Peng, Li Wei, Ding Zhi, Deng Yang, Liu Yan, Zhu Xuerui, Cai Tingting, Li Jianlin, Zheng Tiesong
Department of Food Science and Engineering, Nanjing Normal University, Nanjing, 210024, China.
Department of Electronic and Electrical Engineering, The University of Sheffield, Sheffield, S3 7HQ, United Kingdom.
Anal Biochem. 2018 Aug 1;554:28-33. doi: 10.1016/j.ab.2018.05.025. Epub 2018 May 31.
We designed a competitive aptamer chemiluminescence assay for ochratoxin A (OTA) on the surface of a single silica photonic crystal microsphere (SPCM) in cereal samples. The structural color of SPCMs is used to recognize and trace the microspheres during process of detection. Anti-aptamer was immobilized on the surface of SPCM. OTA and anti-aptamer competed to bind to aptamer when OTA and its aptamer (labeled by biotin at 5'end) were added in the system. The chemiluminescence signal was developed by the horseradish peroxidase (HRP), luminol and HO. The molecules on the single SPCM can produce enough chemiluminescence signal intensity for quantitative detection for OTA. The linear detection range for OTA was from 1 pg/mL to 1 ng/mL and recovery rates were 89%-95%, 81%-92% and 94%-105% in rice, wheat and corn, respectively. The results showed that the developed method for OTA using a single SPCM has a great application potential in cereal samples.
我们设计了一种用于检测谷物样品中赭曲霉毒素A（OTA）的竞争性适体化学发光分析法，该方法基于单个二氧化硅光子晶体微球（SPCM）表面。在检测过程中，利用SPCM的结构颜色来识别和追踪微球。将抗适体固定在SPCM表面。当系统中加入OTA及其适体（5'端用生物素标记）时，OTA和抗适体竞争结合适体。通过辣根过氧化物酶（HRP）、鲁米诺和H₂O₂产生化学发光信号。单个SPCM上的分子能够产生足够的化学发光信号强度用于OTA的定量检测。OTA的线性检测范围为1 pg/mL至1 ng/mL，在大米、小麦和玉米中的回收率分别为89% - 95%、81% - 92%和94% - 105%。结果表明，所开发的基于单个SPCM的OTA检测方法在谷物样品中具有很大的应用潜力。
