Institute of Virology, Leipzig University, Leipzig, Germany.
Institute of Virology, Leipzig University, Leipzig, Germany.
Infect Genet Evol. 2018 Sep;63:180-194. doi: 10.1016/j.meegid.2018.05.030. Epub 2018 May 31.
Species A rotaviruses are highly diverse and impose a substantial burden to human and animal health. Interspecies transmission between livestock, domestic animals and humans is commonly observed, but spread of animal-like rotaviruses within the human population is limited. During the continued monitoring of rotavirus strains in Germany, an unusual G6P[9] rotavirus strain was detected in feces of a child. The complete rotavirus coding sequences revealed a unique G6-P[9]-I2-R2-C2-M2-A3-N2-T3-E2-H3 genotype constellation. The virus was phylogenetically related to feline G3P[9] strains and other human G6P[9] rotaviruses of presumable zoonotic origin. Analysis of primer binding sites of G6 specific genotyping revealed further evidence of a G6P[9] feline reservoir. Moreover, substantial deficits of conventional semi-nested PCR genotyping approaches in detecting contemporary G6P[9] were revealed. Rotavirus strain GER29-14 most likely resulted from a direct or recent interspecies transmission from a cat to human. Further studies could assess nucleic acid sequences and genotype constellations of feline rotavirus to confirm the likely feline origin of sporadic human G6P[9] strains.
种 A 轮状病毒具有高度多样性，对人类和动物健康造成了巨大负担。家畜、家禽和人类之间的种间传播很常见，但动物样轮状病毒在人群中的传播是有限的。在德国持续监测轮状病毒株的过程中，从一名儿童的粪便中检测到一种不寻常的 G6P[9]轮状病毒株。完整的轮状病毒编码序列显示出独特的 G6-P[9]-I2-R2-C2-M2-A3-N2-T3-E2-H3 基因型组合。该病毒与猫科动物 G3P[9]株和其他可能具有动物源性的人类 G6P[9]轮状病毒在系统进化上有关。对 G6 特异性基因分型的引物结合位点的分析进一步证明了 G6P[9]的猫科动物储存库。此外，还发现传统的半巢式 PCR 基因分型方法在检测当代 G6P[9]方面存在严重缺陷。GER29-14 轮状病毒株很可能是猫与人之间直接或近期种间传播的结果。进一步的研究可以评估猫科动物轮状病毒的核酸序列和基因型组合，以确认散发性人类 G6P[9]株的可能猫科动物起源。
