Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.
School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia.
Anim Reprod Sci. 2018 Aug;195:216-229. doi: 10.1016/j.anireprosci.2018.05.026. Epub 2018 May 30.
Recently, there has been a growing interest in the ovarian maturation of mud crabs, genus Scylla. Studies regarding the factors that affect ovarian maturation in mud crabs, however, are still lacking. This study, therefore, evaluates the relationship between diet and internal physiological changes of female orange mud crabs, Scylla olivacea. Sixty female adult S. olivacea were sampled from Setiu Wetland, Malaysia. Foreguts were sampled to study fullness and content. The hepatopancreas was sampled to study digestive enzyme activity, biochemical composition, and histology. Ovaries were sampled to study ovarian biochemical composition and histology. Foregut fullness data suggest that there is an increase in feeding with advancing ovarian maturation in mud crabs. Data for foregut contents indicated that when the ovary was in Stage 3 of maturation the diet was animal-based. The activity of amylase, cellulase, lipase, and trypsin during Stages 1 and 4 of ovarian maturation were less than during Stage 3 of ovarian maturation. Biochemical composition (protein and lipid) was greater during Stage 3 of ovarian maturation. Histological analysis of the hepatopancreas indicated an increase in hepatopancreas tubules, B and R cells during Stage 3 as compared with Stage 1 of ovarian maturation. Histological analysis of the ovary indicated increases in oocyte diameter due to concentrated large yolk globules. Based on these results, it is concluded that vitellogenesis mainly occurred during Stage 3 of ovarian maturation, based on evidence of increased feeding with the diet being predominantly animal-based. Likewise, digestive enzyme activities, proteins, lipids, B cells, and R cells were all greater during Stage 3 of ovarian maturation.
最近，人们对泥蟹属 Scylla 的卵巢成熟度越来越感兴趣。然而，关于影响泥蟹卵巢成熟的因素的研究仍然缺乏。因此，本研究评估了饮食与雌性橙泥蟹 Scylla olivacea 内部生理变化之间的关系。从马来西亚塞蒂乌湿地采集了 60 只成年雌性 S. olivacea。采集前肠以研究饱满度和内容物。采集肝胰腺以研究消化酶活性、生化成分和组织学。采集卵巢以研究卵巢生化成分和组织学。前肠饱满度数据表明，随着卵巢成熟度的提高，泥蟹的摄食量增加。前肠内容物数据表明，当卵巢处于成熟第 3 期时，饮食以动物为基础。在卵巢成熟的第 1 期和第 4 期，淀粉酶、纤维素酶、脂肪酶和胰蛋白酶的活性低于第 3 期卵巢成熟时的活性。生化成分（蛋白质和脂质）在卵巢成熟第 3 期较高。肝胰腺组织学分析表明，与卵巢成熟第 1 期相比，第 3 期肝胰腺小管、B 和 R 细胞增加。卵巢组织学分析表明，卵母细胞直径因浓缩的大卵黄球而增加。基于这些结果，可以得出结论，基于摄食增加的证据，主要发生在卵巢成熟第 3 期的卵黄发生，而饮食主要以动物为基础。同样，在卵巢成熟第 3 期，消化酶活性、蛋白质、脂质、B 细胞和 R 细胞均较高。
