Duangprom Supawadee, Ampansri Wilailuk, Suwansa-Ard Saowaros, Chotwiwatthanakun Charoonroj, Sobhon Prasert, Kornthong Napamanee
Chulabhorn International Collage of Medicine, Thammasat University, Rangsit Campus, Pathumthani, Thailand.
Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
Anim Reprod Sci. 2018 Nov;198:220-232. doi: 10.1016/j.anireprosci.2018.09.022. Epub 2018 Sep 29.
Prostaglandins have important physiological roles in marine invertebrates, including larval development and reproduction. The prostaglandin E concentration fluctuates during the ovarian development of crustaceans. The biosynthetic pathway of prostaglandin, however, has not been well studied in portunid crabs, including in the mud crab, Scylla olivacea. In this study, the aim was to investigate the presence of prostaglandin E synthase (PGES), enzyme that catalyzes the terminal conversion in the prostaglandin E2 (PGE) biosynthesis, and its gene expression in the central nervous system (CNS) and ovary during ovarian maturation of S. olivacea. cDNA sequence encoding PGES was cloned from the S. olivacea ovary. The PGES transcript of S. olivacea (Scyol-PGES) consists of 1258 nucleotides, which encodes for 420 amino acid PGES protein precursor. Investigation of gene expression by RT-PCR indicated that Scyol-PGES was detected in all organs studied. Based on in situ hybridization, Scyol-PGES was detected in the I to III stages for oocyte development of Stage 3 of ovarian development, and in the CNS, including the various neuronal clusters of the brain. In the ventral nerve cord, the Scyol-PGES gene was expressed in the neurons within the subesophageal, thoracic and abdominal ganglia. The Scyol-PGES gene expression as indicated by relative abundance of mRNA in the Stage 4 of ovarian development was greater than that at Stages 1 to 3 of ovarian development. This is the first report on PGES in the mud crab, S. olivacea, and its gene expression suggested the involvement of PGES in the ovarian development of this species.
前列腺素在海洋无脊椎动物中具有重要的生理作用，包括幼体发育和繁殖。在甲壳类动物的卵巢发育过程中，前列腺素E的浓度会发生波动。然而，在梭子蟹科螃蟹，包括榄绿青蟹（Scylla olivacea）中，前列腺素的生物合成途径尚未得到充分研究。在本研究中，目的是调查前列腺素E合酶（PGES）的存在情况，PGES是一种催化前列腺素E2（PGE）生物合成中末端转化的酶，以及其在榄绿青蟹卵巢成熟过程中在中枢神经系统（CNS）和卵巢中的基因表达。从榄绿青蟹卵巢中克隆了编码PGES的cDNA序列。榄绿青蟹的PGES转录本（Scyol-PGES）由1258个核苷酸组成，编码420个氨基酸的PGES蛋白前体。通过RT-PCR对基因表达的研究表明，在所有研究的器官中都检测到了Scyol-PGES。基于原位杂交，在卵巢发育第3阶段卵母细胞发育的I至III期以及在中枢神经系统，包括大脑的各种神经簇中检测到了Scyol-PGES。在腹神经索中，Scyol-PGES基因在咽下神经节、胸神经节和腹神经节内的神经元中表达。卵巢发育第4阶段中mRNA的相对丰度表明，Scyol-PGES基因表达高于卵巢发育第1至3阶段。这是关于榄绿青蟹中PGES的首次报道，其基因表达表明PGES参与了该物种的卵巢发育。
