爱泼斯坦-巴尔病毒EBER转录本影响miRNA介导的特定靶标的调控，并被加工成小RNA种类。
Epstein-Barr Virus EBER Transcripts Affect miRNA-Mediated Regulation of Specific Targets and Are Processed to Small RNA Species.
作者信息
Alles Julia, Hasler Daniele, Kazmi Syed Mohammad Ali, Tesson Mathias, Hamilton Andrew, Schlegel Linda, Marx Stefanie, Eichner Norbert, Reinhardt Richard, Meister Gunter, Wilson Joanna B, Grässer Friedrich A
机构信息
Institute of Virology, Saarland University Medical School, Kirrbergerstrasse, Haus 47, Homburg/Saar D-66421, Germany.
Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, Universitätsstrasse 31, Regensburg D-93053, Germany.
出版信息
Noncoding RNA. 2015 Sep 30;1(3):170-191. doi: 10.3390/ncrna1030170.
The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5'-derived RNAs of approximately 23, 52 and 70 nt (EBER123, EBER152, and EBER170) and of EBER2 to 3' fragments. The biogenesis of these species is independent of Dicer, and EBER123 does not act like a miRNA OPEN ACCESS Non-Coding RNA 2015, 1 171 to target its complementary sequence. EBER1, EBER2 and EBER123 were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.
致癌性爱泼斯坦-巴尔病毒（EBV）表达44种成熟的微小RNA以及两种长度分别为167个核苷酸（EBER1）和172个核苷酸（EBER2）的非编码EBER RNA。对NK/T细胞系和原代细胞进行微小RNA分析，以及对EBV感染的细胞系和原发性肿瘤进行Northern印迹分析，结果显示EBER1可加工成约23、52和70个核苷酸的5'端短衍生RNA（EBER123、EBER152和EBER170），EBER2可加工成3'端片段。这些RNA的生物合成不依赖于Dicer，并且EBER123的作用不像微小RNA那样靶向其互补序列。EBER1、EBER2和EBER123与狼疮抗原（La）结合，La是一种促进RNA干扰的核质蛋白。与此一致的是，EBER会影响携带微小RNA靶序列的白细胞介素1α（IL1α）和RAC1报告基因的调控，这些靶序列是miR-142-3p的靶标。然而，EBER对miR-142-3p的另一个靶标ADCY9以及ebv-miR-BART16的靶标TOMM22均无影响，这表明EBER对基因表达具有选择性调控作用。
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