Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine "King Michael I" of Banat Timişoara, Calea Aradului 119, 300645 Timişoara, Romania.
Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodenja 18, 11000 Belgrade, Serbia.
Molecules. 2018 May 26;23(6):1278. doi: 10.3390/molecules23061278.
The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves.
Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, α1-globulin, α2-globulin, β-globulin and γ-globulin) were analyzed by electrophoresis on cellulose acetate.
At hour 30 after birth, concentrations of γ-globulins, β-globulin and total protein in E1 group of calves were higher than in control group by 42.11% ( < 0.05), 28.48% ( > 0.05) and 18.52% ( > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, < 0.05 and 35.87%, < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%.
本研究的目的是确定在初乳中添加沸石对新生牛血清蛋白电泳图谱的影响。
研究中涉及的罗马尼亚黑白花新生牛分为 3 组：对照组（C），接受未添加沸石的初乳；实验组 I（E1）和 II（E2），分别接受添加 0.5%和 2%沸石的初乳。通过醋酸纤维素电泳分析总蛋白和蛋白馏分（白蛋白、α1-球蛋白、α2-球蛋白、β-球蛋白和γ-球蛋白）的浓度。
出生后 30 小时，E1 组小牛的γ-球蛋白、β-球蛋白和总蛋白浓度分别比对照组高 42.11%（<0.05）、28.48%（>0.05）和 18.52%（>0.05），E2 组与对照组相比虽有升高，但无统计学意义。这与 E1 和 E2 组的白蛋白/球蛋白比值显著降低（30 小时产后分别为 29.35%（<0.05）和 35.87%（<0.05））相一致，表明实验组球蛋白馏分明显增加。
沸石能有效改善新生牛的被动转移，但在初乳中添加 0.5%的剂量比添加 2%的剂量更有效。
