AHPL/AYTOP/0213乳膏的抗炎和抗菌活性评估。

Evaluation of anti-inflammatory and antimicrobial activity of AHPL/AYTOP/0213 cream.

作者信息

Nipanikar Sanjay U, Nagore Dheeraj, Chitlange Soham S, Buzruk Devashree

机构信息

R and D Center, Ari Healthcare Private Limited, Unit No. 401, International Biotech Park, BTS 2 Building, Chrysalis Enclave, 4 Floor, Plot No - 2A, MIDC Phase II, Pimpri, Pune, Maharashtra, India.

Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, India.

出版信息

Ayu. 2017 Jan-Jun;38(1-2):82-87. doi: 10.4103/ayu.AYU_150_17.

DOI:10.4103/ayu.AYU_150_17

PMID:29861599

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5954256/

Abstract

BACKGROUND

Acne vulgaris is almost a widespread disease occurring in all races. initiate acne and inflammatory mediators aggravate it. Conventional therapies for acne include comedolytic, anti-inflammatory, and anti-biotic agents. Due to adverse effects of these therapies, people are searching for alternative options. In this context, a polyherbal formulation AHPL/AYTOP/0213 cream was developed for the treatment of Acne.

OBJECTIVE

The objective of this study is to study anti-inflammatory and antimicrobial activities of AHPL/AYTOP/0213 cream.

MATERIALS AND METHODS

Skin irritation study was conducted on AHPL/AYTOP/0213 cream as per OECD guidelines. (1) Anti-inflammatory activity: Anti-inflammatory activity of AHPL/AYTOP/0213 cream in comparison with diclofenac sodium cream was assessed in carrageenan-induced rat paw edema model. (2) Antimicrobial activity for . : . were incubated under anaerobic conditions. Aliquots of molten brain-heart infusion with glucose agar were used as the agar base. Formulation and clindamycin (10 mg/ml) were introduced in to the Agar wells randomly. (3) Antimicrobial activity for and : bacteria were incubated under aerobic conditions at 37°C. Tryptic soy broth with glucose agar was used as the agar base. A volume of 0.5 ml of formulation and clindamycin (10 mg/ml) were introduced in to the wells randomly. The antibacterial activity was evaluated by measuring zones of inhibition (in mm).

RESULTS

AHPL/AYTOP/0213 cream is nonirritant. Significant reduction in rat paw edema (43%) was observed with AHPL/AYTOP/0213 which was also comparable to diclofenac sodium cream (56.09%). Zone of inhibition for formulation was 20.68 mm, 28.20 mm, and 21.40 mm for . , . and . , respectively, which was comparable to clindamycin. The minimum inhibitory concentration of formulation AHPL/AYTOP/0213 obtained in anti-microbial study was 2.5 mg/mL.

CONCLUSION

AHPL/AYTOP/0213 cream is nonirritant and possesses significant anti-inflammatory and antimicrobial activities, which further justifies its role in the management of acne vulgaris.

摘要

背景

寻常痤疮几乎是一种在所有种族中都普遍存在的疾病。引发痤疮的因素及炎症介质会加重病情。痤疮的传统治疗方法包括角质溶解剂、抗炎药和抗生素。由于这些治疗方法存在不良反应，人们正在寻找替代方案。在此背景下，开发了一种多草药配方AHPL/AYTOP/0213乳膏用于治疗痤疮。

目的

本研究的目的是研究AHPL/AYTOP/0213乳膏的抗炎和抗菌活性。

材料与方法

按照经合组织指南对AHPL/AYTOP/0213乳膏进行皮肤刺激性研究。（1）抗炎活性：在角叉菜胶诱导的大鼠足肿胀模型中评估AHPL/AYTOP/0213乳膏与双氯芬酸钠乳膏相比的抗炎活性。（2）对……的抗菌活性：……在厌氧条件下培养。含有葡萄糖琼脂的融化脑心浸液等分试样用作琼脂基质。将配方制剂和克林霉素（10mg/ml）随机加入琼脂孔中。（3）对……和……的抗菌活性：细菌在37℃有氧条件下培养。含有葡萄糖琼脂的胰蛋白胨大豆肉汤用作琼脂基质。将0.5ml配方制剂和克林霉素（10mg/ml）随机加入孔中。通过测量抑菌圈（以毫米为单位）评估抗菌活性。

结果

AHPL/AYTOP/0213乳膏无刺激性。AHPL/AYTOP/0213乳膏使大鼠足肿胀明显减轻（4?3%），这也与双氯芬酸钠乳膏（56.09%）相当。配方制剂对……、……和…… 的抑菌圈分别为20.68mm、28?20mm和21.40mm，与克林霉素相当。在抗菌研究中获得的AHPL/AYTOP/0213配方制剂的最低抑菌浓度为2.5mg/mL。