Health Sciences, California Northstate University, Rancho Cordova, CA, USA.
GeneScan Diagnostics, LLC., San Diego, CA, USA.
Biochem Biophys Res Commun. 2022 Sep 24;622:45-49. doi: 10.1016/j.bbrc.2022.06.068. Epub 2022 Jun 30.
Although evidence supports that the acne microbiome harbors a diverse range of microbes that play a vital role in the progression of acne vulgaris, the culturable microbes in the acne microbiome have not yet been largely identified. Here, we grew microbe colonies from entire acne lesions on agar plates and identified abundant Staphylococcus, Acinetobacter, and Pseudomonas species from forty selected single colonies. Staphylococcus species, including Staphylococcus epidermidis (S. epidermidis), Staphylococcus hominis (S. hominis), and Staphylococcus aureus (S. aureus), were isolated from tryptic soy broth (TSB) agar plates. However, Cutibacterium acnes (C. acnes) was predominately isolated from furazolidone-supplemented TSB agar plates. Results from gas chromatography-mass spectrometry (GC-MS) analysis revealed that, besides acetate, propionate and butyrate were the main short-chain fatty acids (SCFAs) in fermentation metabolites of C. acnes and S. epidermidis isolates, respectively. The culturable bacteria and SCFA profiles presented in this study provide a reservoir for selecting acne probiotics and developing SCFA-associated therapies against acne vulgaris.
尽管有证据表明痤疮微生物组中存在着多种微生物，这些微生物在寻常痤疮的发展中起着至关重要的作用，但痤疮微生物组中的可培养微生物尚未得到广泛鉴定。在这里，我们从琼脂平板上的整个痤疮病变中培养微生物菌落，并从四十个选定的单个菌落中鉴定出丰富的葡萄球菌、不动杆菌和假单胞菌属。从胰蛋白酶大豆肉汤（TSB）琼脂平板上分离出葡萄球菌属，包括表皮葡萄球菌（S. epidermidis）、人葡萄球菌（S. hominis）和金黄色葡萄球菌（S. aureus）。然而，痤疮丙酸杆菌（C. acnes）主要从呋喃唑酮补充的 TSB 琼脂平板上分离出来。气相色谱-质谱（GC-MS）分析结果表明，除了乙酸外，丙酸和丁酸分别是痤疮丙酸杆菌和表皮葡萄球菌分离株发酵代谢物中的主要短链脂肪酸（SCFA）。本研究中可培养细菌和 SCFA 图谱为选择痤疮益生菌和开发与 SCFA 相关的治疗寻常痤疮的方法提供了一个储备。
