3-甲基吲哚对山羊肺微粒体超氧化物和过氧化氢生成以及NADPH氧化的影响。

The effect of 3-methylindole on superoxide and hydrogen peroxide production and NADPH oxidation by goat lung microsomes.

作者信息

Laegreid W W, Breeze R G

出版信息

Res Commun Chem Pathol Pharmacol. 1985 Mar;47(3):387-97.

Abstract

The production of superoxide and hydrogen peroxide and the oxidation of NADPH by goat lung microsomes in the presence of 3MI or nitrofurantoin were investigated. Although nitrofurantoin strongly enhanced microsomal formation of adrenochrome from epinephrine (a superoxide dependent reaction), 3MI did not increase the rate of adrenochrome formation by microsomes. Neither 3MI nor nitrofurantoin stimulated significant increases in microsomal hydrogen peroxide production. 3MI tripled NADPH oxidation compared to controls, while nitrofurantoin caused a nearly twenty-fold increase in NADPH oxidation. It is concluded that production of superoxide, hydrogen peroxide and depletion of intracellular stores of reduced pyridine nucleotides are not significant components of 3MI pneumotoxicity.

摘要

研究了在3-甲基吲哚（3MI）或呋喃妥因存在下，山羊肺微粒体中超氧化物和过氧化氢的产生以及NADPH的氧化情况。尽管呋喃妥因强烈增强了微粒体由肾上腺素生成肾上腺色素的过程（一种依赖超氧化物的反应），但3MI并未提高微粒体生成肾上腺色素的速率。3MI和呋喃妥因均未刺激微粒体过氧化氢产量的显著增加。与对照组相比，3MI使NADPH氧化增加了两倍，而呋喃妥因使NADPH氧化增加了近二十倍。得出的结论是，超氧化物的产生、过氧化氢的产生以及还原型吡啶核苷酸细胞内储存的消耗并非3MI肺毒性的重要组成部分。