Department of Veterinary Medicine and Animal Production , University of Napoli "Federico II" , Via Delpino 1 , 80137 Napoli , Italy.
Department of Science , University of Basilicata , Viale dell'Ateneo Lucano 10 , 85100 Potenza , Italy.
J Agric Food Chem. 2018 Jun 27;66(25):6388-6393. doi: 10.1021/acs.jafc.8b00229. Epub 2018 Jun 13.
The purpose of this research was to evaluate the persistence of penicillin G and dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin G (200000 IU/mL) and dihydrostreptomycin sulfate (250 mg/mL) every 24 h for 3 days. Milk samples were collected twice daily up to the 13th milking post-treatment and analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. The analytical method has been validated according to Commission Decision 2002/657/EC. The highest concentrations of penicillin G (275 μg kg) and dihydrostreptomycin (220.5 μg kg) were detected in the milk of the first milkings post-treatment, and levels were below the maximum residue limit of 4 and 200 μg kg in all treated buffaloes at milkings 12 and 2, respectively. The results of this study demonstrate that a nine-milking withdrawal time set for bovine milk was not adequate for depletion of penicillin G in lactating buffaloes.
本研究旨在评估普鲁卡因青霉素 G（200000IU/mL）和硫酸双氢链霉素（250mg/mL）肌内注射，每 24 小时注射 3 天，在哺乳期水牛乳中的青霉素 G 和双氢链霉素的持久性。在治疗后第 13 次挤奶前每天采集两次牛奶样本，并通过超高效液相色谱-串联质谱法进行分析。该分析方法已经按照欧盟委员会 2002/657/EC 号决定进行了验证。在治疗后的第一次挤奶中，青霉素 G（275μg/kg）和双氢链霉素（220.5μg/kg）的浓度最高，并且在所有接受治疗的水牛的第 12 和第 2 次挤奶中，浓度均低于 4μg/kg 和 200μg/kg 的最大残留限量。本研究结果表明，牛乳中设定的 9 次挤奶停药期不足以使哺乳期水牛体内的青霉素 G 耗尽。
