Zhang Liwen, Bai Yongliang, Su Shulan, Ouyang Zhen, Liu Li, Pan Gang, Qian Dawei, Duan Jinao
a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization , Nanjing University of Chinese Medicine , Nanjing , China.
b School of Pharmaceutics , Jiangsu University , Zhenjiang , China.
Nat Prod Res. 2019 Apr;33(8):1182-1190. doi: 10.1080/14786419.2018.1465422. Epub 2018 Jun 4.
Metabolism, transformation and dynamic changes of DNJ, 2-O-α-D-Gal-DNJ, fagomine, isofagomine and 4-O-β-d-Glc-fagomine from mulberry leaves in silkworms at different instars were observed. UPLC-Q/TOF-MS and UPLC-TQ/MS methods were adopted for qualitative and quantitative analysis respectively. Three species mulberry leaves were used to feed the silkworm as controls. By analyzing and comparing the content changes of DNJ, fagomine and their derivatives in silkworms and silkworm excrement at different instar, we revealed the dynamic changes, confirmed the enrichment effect of the polyhydroxy alkaloids by silkworm, and inferred the conversion process behind this effect. The experimental results indicated that DNJ and its derivatives turned into some intermediate substances in the metabolic process, and finally they converted back and the content increased. Fagomine and its derivatives interconverted into each other in the process, 4-O-β-d-Glc-fagomine transformed into fagomine, while fagomine transformed into isofagomine.
观察了不同龄期家蚕体内桑叶中1-脱氧野尻霉素（DNJ）、2-O-α-D-半乳糖-DNJ、1,4-二脱氧野尻霉素、异1,4-二脱氧野尻霉素和4-O-β-D-葡萄糖-1,4-二脱氧野尻霉素的代谢、转化及动态变化。分别采用超高效液相色谱-四极杆/飞行时间质谱（UPLC-Q/TOF-MS）和超高效液相色谱-三重四极杆质谱（UPLC-TQ/MS）方法进行定性和定量分析。以三种桑叶作为对照来饲养家蚕。通过分析比较不同龄期家蚕及蚕粪中DNJ、1,4-二脱氧野尻霉素及其衍生物的含量变化，揭示了其动态变化规律，证实了家蚕对多羟基生物碱的富集作用，并推断出该作用背后的转化过程。实验结果表明，DNJ及其衍生物在代谢过程中转化为一些中间物质，最终又转化回来且含量增加。1,4-二脱氧野尻霉素及其衍生物在该过程中相互转化，4-O-β-D-葡萄糖-1,4-二脱氧野尻霉素转化为1,4-二脱氧野尻霉素，而1,4-二脱氧野尻霉素转化为异1,4-二脱氧野尻霉素。
