采用亲水作用色谱-串联质谱法检测桑叶中肌醇糖的季节性变化。

Seasonal variations of iminosugars in mulberry leaves detected by hydrophilic interaction chromatography coupled with tandem mass spectrometry.

机构信息

Hygiene Detection Center, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China.

出版信息

Food Chem. 2018 Jun 15;251:110-114. doi: 10.1016/j.foodchem.2018.01.058. Epub 2018 Jan 9.

DOI:10.1016/j.foodchem.2018.01.058

PMID:29426416

Abstract

A rapid, sensitive, and validated method was developed for the simultaneous determination of four iminosugars, 1-deoxynojirimycin (DNJ), d-fagomine (FAG), 2-O-a-d-galactopyranosyl-DNJ (Gal-DNJ), and 4-O-β-d-glucopyranosyl-fagomine (Glu-FAG), in mulberry leaves. The method used hydrophilic interaction chromatography coupled with tandem mass spectrometry. Based on this method, the seasonal variations of iminosugars in the leaves of different mulberry species (Morus alba, Morus multicaulis Perr, Morus atropurpurea Roxb. and Morus wittiorum Hand-Mazz.) collected in Guangzhou, China, during 10 consecutive months in 2013 were investigated. The results indicated that the maximum content of DNJ in the leaves of all four species occurred in summer (June or July). The highest FAG level was found in spring (April or May). The highest levels of Gal-DNJ and Glu-FAG were recorded in autumn (September or October).

摘要

建立了一种快速、灵敏、有效的同时测定桑叶中 4 种氨基糖的方法，包括 1-脱氧野尻霉素（DNJ）、d-松二糖（FAG）、2-O-α-d-吡喃半乳糖基-DNJ（Gal-DNJ）和 4-O-β-d-吡喃葡萄糖基-FAG（Glu-FAG）。该方法采用亲水相互作用色谱-串联质谱法。基于该方法，研究了 2013 年 10 个月间在中国广州采集的不同桑种（白桑、多脉桑、蒙桑和华桑）叶片中氨基糖的季节性变化。结果表明，4 种桑属植物叶片中 DNJ 的最大含量均出现在夏季（6 月或 7 月），FAG 的最高含量出现在春季（4 月或 5 月），Gal-DNJ 和 Glu-FAG 的最高含量出现在秋季（9 月或 10 月）。