建立红平红球菌N9T-4的有效贫营养培养系统。
Establishment of an effective oligotrophic cultivation system for Rhodococcus erythropolis N9T-4.
作者信息
Matsuoka Tomohiro, Yoshida Nobuyuki
机构信息
a Applied Chemistry and Biochemical Engineering Course, Department of Engineering , Graduate School of Integrated Science and Technology, Shizuoka University , Hamamatsu , Japan.
出版信息
Biosci Biotechnol Biochem. 2018 Sep;82(9):1652-1655. doi: 10.1080/09168451.2018.1482196. Epub 2018 Jun 3.
Rhodococcus erythropolis N9T-4 grows on an inorganic solid-state medium with no additional carbon and energy sources; however, it is unable to grow well in a liquid culture medium under the oligotrophic conditions. We examined submerged cultivations of N9T-4 using a polyurethane foam sponge to achieve approximately 10 times of the oligotrophic growth of the bacterium in the liquid culture medium.
红平红球菌N9T-4能在不添加额外碳源和能源的无机固态培养基上生长；然而，在贫营养条件下，它在液体培养基中无法良好生长。我们使用聚氨酯泡沫海绵对N9T-4进行了深层培养，以使该细菌在液体培养基中的贫营养生长量达到约10倍。
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