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一种用于扩增富含DHA的微藻——金藻属线粒体细胞色素C氧化酶亚基I（COI）基因的新型引物组。

A New Primer Set to Amplify the Mitochondrial Cytochrome C Oxidase Subunit I (COI) Gene in the DHA-Rich Microalgae, the Genus Aurantiochytrium.

作者信息

Nishitani Goh, Yoshida Masaki

机构信息

Graduate School of Agricultural Science, Tohoku University.

Faculty of Life and Environmental Sciences, University of Tsukuba.

出版信息

Microbes Environ. 2018 Jul 4;33(2):227-229. doi: 10.1264/jsme2.ME17145. Epub 2018 Jun 1.

DOI:10.1264/jsme2.ME17145
PMID:29863058
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6031396/
Abstract

This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

摘要

本研究旨在开发一套针对富含二十二碳六烯酸（DHA）的金藻属微藻线粒体细胞色素c氧化酶亚基I（COI）的引物组。使用12株金藻菌株和其他破囊壶菌物种对该引物组的性能进行了测试。与核18S rRNA基因序列不同，金藻菌株的线粒体序列没有遗传多态性。新开发的这套引物组扩增出了来自金藻属及其近缘属的序列，可能有助于该领域中金藻属的物种鉴定和遗传多样性的阐明。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/6031396/d01fb86540c7/33_227_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/6031396/d5eecf459ea8/33_227_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/6031396/d01fb86540c7/33_227_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/6031396/d5eecf459ea8/33_227_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5d4/6031396/d01fb86540c7/33_227_2.jpg

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