Laboratory of Pharmaceutical and Medicinal Chemistry, Gifu Pharmaceutical University, Japan.
Metallomics. 2018 Jun 20;10(6):794-801. doi: 10.1039/c8mt00049b.
Mitochondria are iron-rich organelles that are involved in the process of energy production through the electron-transporting system and heme synthesis. We developed a new mitochondria-targeted fluorescent probe, MtFluNox/Ac-MtFluNox, for Fe(ii) based on N-oxide chemistry, which we recently established as a Fe(ii)-selective fluorogenic switch. The deacetylated form MtFluNox showed a turn-on response towards Fe(ii) with high metal selectivity in cuvette experiments, and an imaging study using its cell-compatible analogue Ac-MtFluNox demonstrated mitochondria-specific fluorescence enhancement in response to Fe(ii) in living cells. Furthermore, the probe was able to detect endogenously accumulated Fe(ii) induced as a result of the inhibition of heme synthesis.
线粒体是富含铁的细胞器，通过电子传递系统和血红素合成参与能量产生的过程。我们基于氮氧化物化学开发了一种新的线粒体靶向荧光探针 MtFluNox/Ac-MtFluNox，用于检测 Fe(ii)，这是我们最近建立的一种 Fe(ii)选择性荧光开关。去乙酰化形式的 MtFluNox 在试管实验中对 Fe(ii)表现出开环响应，具有高金属选择性，并且使用其细胞相容类似物 Ac-MtFluNox 的成像研究表明，在活细胞中，该探针能够检测到血红素合成抑制导致的内源性积累的 Fe(ii)。
