Department of Orthopedics, The First People's Hospital of Changzhou, Changzhou, China.
Eur Rev Med Pharmacol Sci. 2018 May;22(10):2966-2972. doi: 10.26355/eurrev_201805_15051.
To investigate the expression of long non-coding RNA (lncRNA) FAS-AS1 in osteoarthritis cartilage and to explore its effect on articular cartilage cells.
A total of 20 tissue samples of primary knee joint osteoarthritis and 20 tissue samples of knee joint cartilage after traumatic amputation were collected. Fluorescence quantitative polymerase chain reaction (PCR) was performed to detect the expression of FAS-AS1, MMP1, MMP13, and COL2A1 in cartilage. FAS-AS1 small interfering RNA (siRNA) was transfected to chondrocytes transiently to observe its effects on proliferation, apoptosis of chondrocytes, and the expressions of MMP1, MMP13, and COL2A1.
The expressions of FAS-AS1, MMP1, and MMP13 in osteoarthritis tissues increased significantly, while COL2A1 presented a low expression. Reducing the expression of FAS-AS1 inhibited cell apoptosis and promote cell proliferation. Additionally, in vitro experiments showed that low expression of FAS-AS1 decreased the expressions of MMP1 and MMP13, but increased the expression of COL2A1.
The expression of FAS-AS1 was increased in osteoarthritis, and FAS-AS1 could be involved in the development of the disease by regulating the proliferation, apoptosis of chondrocytes and promoting the degradation of extracellular matrix.
研究长链非编码 RNA（lncRNA）FAS-AS1 在骨关节炎软骨中的表达，并探讨其对关节软骨细胞的影响。
收集 20 例原发性膝关节骨关节炎组织样本和 20 例创伤性截肢膝关节软骨组织样本。采用荧光定量聚合酶链反应（PCR）检测软骨中 FAS-AS1、MMP1、MMP13 和 COL2A1 的表达。转染 FAS-AS1 小干扰 RNA（siRNA）瞬时转染软骨细胞，观察其对软骨细胞增殖、凋亡以及 MMP1、MMP13 和 COL2A1 表达的影响。
骨关节炎组织中 FAS-AS1、MMP1 和 MMP13 的表达明显增加，而 COL2A1 表达较低。降低 FAS-AS1 的表达抑制细胞凋亡并促进细胞增殖。此外，体外实验表明，FAS-AS1 低表达降低了 MMP1 和 MMP13 的表达，但增加了 COL2A1 的表达。
FAS-AS1 在骨关节炎中表达增加，FAS-AS1 可能通过调节软骨细胞的增殖、凋亡和促进细胞外基质降解来参与疾病的发生。
