miR-431 通过抑制 CUL4B 抑制结直肠癌细胞侵袭。
MiR-431 inhibits colorectal cancer cell invasion via repressing CUL4B.
机构信息
Department of Gastroenterology, Xiantao First People's Hospital, Xiantao, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 May;22(10):3047-3052. doi: 10.26355/eurrev_201805_15062.
OBJECTIVE
To detect the expression of microRNA-431 (miR-431) in epithelial-mesenchymal transition (EMT) in colorectal carcinoma and investigate its effects on the migration of colorectal cancer cells.
PATIENTS AND METHODS
MiR-431 expressions in colorectal cancer tissues and adjacent tissues were examined via Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The expression levels of cullin-4B (CUL4B) and EMT-related protein were evaluated by Western blotting assay. The invasive ability of the cells was examined via transwell method.
RESULTS
The expression level of miR-431 in colorectal cancer tissues was lower than that in adjacent tissues (p<0.05). After colorectal cancer cell line was transfected with miR-431 mimics, the expression level of e-cadherin was increased in the cells, and the expression levels of n-cadherin, vimentin, fibronectin (FN), and snail were reduced (p<0.05). The migration ability of colorectal cancer cells with miR-431 overexpression was significantly decreased. Moreover, the reverse effect was observed in cells transfected with miR-431 inhibitors.
CONCLUSIONS
The low expression of miR-431 in colorectal cancer patients is involved in the development of colorectal cancer by promoting the migration of colorectal cancer cells.
目的
检测微小 RNA-431（miR-431）在结直肠癌细胞上皮-间质转化（EMT）中的表达，并探讨其对结直肠癌细胞迁移的影响。
方法
采用实时定量逆转录-聚合酶链反应（RT-PCR）检测结直肠癌组织及相邻组织中 miR-431 的表达。采用 Western blot 法检测 CUL4B 和 EMT 相关蛋白的表达水平。通过 Transwell 法检测细胞的侵袭能力。
结果
结直肠癌组织中 miR-431 的表达水平低于相邻组织（p<0.05）。转染 miR-431 模拟物后，结直肠癌细胞中 E-钙黏蛋白的表达水平升高，而 N-钙黏蛋白、波形蛋白、纤维连接蛋白（FN）和 snail 的表达水平降低（p<0.05）。过表达 miR-431 的结直肠癌细胞的迁移能力明显下降。此外，在转染 miR-431 抑制剂的细胞中观察到相反的效果。
结论
结直肠癌患者 miR-431 的低表达通过促进结直肠癌细胞的迁移参与了结直肠癌的发生发展。
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