Center for Process and Systems Engineering (PROSYS), Department of Chemical and Biochemical Engineering, Technical University of Denmark (DTU), Kgs Lyngby DK-2800, Denmark.
Department of Biotechnology, Lund University, Lund SE-22100, Sweden.
Biotechnol Adv. 2018 Nov 15;36(7):1801-1814. doi: 10.1016/j.biotechadv.2018.05.007. Epub 2018 Jun 2.
The application of conventional organic solvents has been essential in several steps of bioprocesses in order to achieve sufficient economic efficiency. The use of organic solvents is frequently used either to partly or fully replace water in the reaction medium or as a process aid for downstream separation. Nowadays, manufacturers are increasingly requested to avoid and substitute solvents with hazardous potential. Therefore, the solvent selection must account for potential environmental hazards, health and safety problems, in addition to fulfilling the ideal characteristics for application in a process. For the first time, criteria including Environment, Health and Safety (EHS), as well as the technical requirements for reaction and separation have been reviewed, collected and integrated in a single organic solvent screening strategy to be used as a guideline for narrowing down the list of solvents to test experimentally. Additionally, we have also included a solvent selection guide based on the methodology developed in the Innovative Medicines Initiative CHEM21 (IMI CHEM21) project and applied specifically to water-immiscible solvents commonly used in bioprocesses.
在生物工艺的多个步骤中，传统有机溶剂的应用对于实现足够的经济效益至关重要。有机溶剂的使用通常用于部分或完全替代反应介质中的水，或作为下游分离的过程助剂。如今，制造商越来越多地被要求避免使用和替代具有潜在危险的溶剂。因此，溶剂选择必须考虑潜在的环境危害、健康和安全问题，除了满足在工艺中应用的理想特性外。这是首次将环境、健康和安全 (EHS) 标准以及反应和分离的技术要求纳入单一的有机溶剂筛选策略中，作为缩小实验测试溶剂范围的指南。此外，我们还根据创新药物倡议 CHEM21（IMI CHEM21）项目中开发的方法学，并专门针对生物工艺中常用的与水不混溶的溶剂，纳入了一个溶剂选择指南。
