Department of Urology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
Department of Urology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, China.
Cancer Biomark. 2018;22(3):543-549. doi: 10.3233/CBM-181202.
Spindle and kinetochore-associated protein 1 (SKA1) is a component of SKA, which is essential for proper chromosome segregation. Recently, SKA1 was found to be over-expressed in several types of human cancers. However, reports on the relationship between SKA1 expression and the prognosis of bladder cancer, in particular, are lacking.
To clarify the clinical significance of SKA1 as a prognostic biomarker for early recurrence and progression of patients with non-muscle invasive bladder cancer (NMIBC).
The differential expression levels of SKA1 of 148 NMIBC tissues were determined by immunohistochemical staining. Quantitative real-time PCR and western blot analysis were further performed to confirm the immunohistochemistry results. Recurrence and progression free interval were assessed by Kaplan-Meier method and differences between groups calculated by log-rank statistics. The prognostic value of SKA1 for early recurrence and progression was analyzed by multivariate Cox proportional hazard regression models.
SKA1 expression was significantly different in various NMIBC tissues. Kaplan-Meier analysis revealed that patients with high SKA1 expression showed high early recurrence (p< 0.001) and progression (p< 0.05) rates. Although univariate Cox regression analysis revealed that several other factors had an impact on recurrence and progression, upon multivariate analysis, high SKA1expression was the only independent predictor for early recurrence (hazards ratio [HR], 0.246; 95% confidence interval [CI], 0.131-0.461; p= 0.000) and progression (HR, 0.194; 95% CI, 0.052-0.715; p= 0.014).
High SKA1 expression is associated with early recurrence and progression in patients with NMIBC, indicating SKA1 may serve as a promising prognostic biomarker for this disease.
纺锤体和着丝粒相关蛋白 1（SKA1）是 SKA 的一个组成部分，对正确的染色体分离至关重要。最近发现 SKA1 在几种类型的人类癌症中过度表达。然而，关于 SKA1 表达与膀胱癌，尤其是非肌层浸润性膀胱癌（NMIBC）患者预后之间的关系的报道尚缺乏。
阐明 SKA1 作为非肌层浸润性膀胱癌（NMIBC）患者早期复发和进展的预后生物标志物的临床意义。
通过免疫组织化学染色检测 148 例 NMIBC 组织中 SKA1 的差异表达水平。进一步进行定量实时 PCR 和 Western blot 分析以验证免疫组织化学结果。通过 Kaplan-Meier 方法评估复发和无进展间隔，通过对数秩统计计算组间差异。通过多变量 Cox 比例风险回归模型分析 SKA1 对早期复发和进展的预后价值。
SKA1 表达在各种 NMIBC 组织中存在显著差异。Kaplan-Meier 分析显示，高 SKA1 表达的患者具有较高的早期复发（p<0.001）和进展（p<0.05）率。虽然单因素 Cox 回归分析显示，其他一些因素对复发和进展有影响，但多因素分析表明，高 SKA1 表达是早期复发（风险比 [HR]，0.246；95%置信区间 [CI]，0.131-0.461；p=0.000）和进展（HR，0.194；95% CI，0.052-0.715；p=0.014）的唯一独立预测因素。
高 SKA1 表达与 NMIBC 患者的早期复发和进展相关，表明 SKA1 可能是该疾病有前途的预后生物标志物。
