Hemopoeitic Stem cell Research Centre (HSCRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Clin Hemorheol Microcirc. 2019;71(1):9-15. doi: 10.3233/CH-170311.
The human scavenger receptor class B type 1 (hSR-B1), which serves as a high affinity receptor for HDL, is expressed on platelet surface and mediates various anti-atherogenic functions. Based on the anti-thrombotic effect of HDL and the importance of HDL-SR-B1 in the formation of atherosclerotic plaque, the present study was aimed to investigate and compare the expression level of hSR-B1on platelets of CAD patients with that of normal controls.
The expression of the hSR-B1 on platelets of 31 CAD patients with atherosclerotic plaque and 20 healthy controls were detected using flowcytometry and western blotting. Moreover, platelet function in response to the agonists was examined by aggregometry, and the lipid panel tests were assayed using chemistry autoanalyzer.
Our findings showed that the expression of hSR-B1 was significantly reduced on the surface of platelets from CAD patients with atherosclerotic disease, as compared with healthy controls (6/8% vs. 13/6%) (P < 0,001). Of particular of interest, we also found that the formation of aggregates after stimulation of the platelets with ADP was higher in patients with atherosclerotic disease than the controls; indicating an inverse relationship between hSR-B1 expression and the function of human platelets.
Taken together, the results of the present study raise the possibility that the measurement of hSR-B1 expression on human platelets may provide a valuable insight that reflects the status of RCT in patients with atherosclerosis.
人清道夫受体 B 类 1 型（hSR-B1）作为高密度脂蛋白（HDL）的高亲和力受体，表达于血小板表面，并介导多种抗动脉粥样硬化功能。基于 HDL 的抗血栓作用以及 HDL-SR-B1 在动脉粥样硬化斑块形成中的重要性，本研究旨在检测和比较冠心病患者血小板 hSR-B1 的表达水平与正常对照者的差异。
采用流式细胞术和 Western blot 检测 31 例冠心病伴动脉粥样硬化斑块患者和 20 例健康对照者血小板 hSR-B1 的表达。此外，通过聚集度测定法检测血小板对激动剂的反应功能，使用化学自动分析仪检测血脂谱。
我们的研究结果表明，与健康对照组（6/8%比 13/6%）相比，冠心病伴动脉粥样硬化患者血小板表面 hSR-B1 的表达显著降低（P < 0.001）。特别值得注意的是，我们还发现，与对照组相比，动脉粥样硬化患者的血小板在 ADP 刺激后形成的聚集物更高，表明 hSR-B1 表达与人类血小板功能呈负相关。
综上所述，本研究结果提示，测量人血小板上 hSR-B1 的表达可能为反映动脉粥样硬化患者的 RCT 状态提供有价值的信息。
