Vieweger Stella E, Tsvetkova Irina B, Dragnea Bogdan G
Chemistry Department, Indiana University, Bloomington, IN, USA.
Methods Mol Biol. 2018;1776:279-294. doi: 10.1007/978-1-4939-7808-3_19.
Nanoparticle-templated assembly of virus shells provides a promising approach to the production of hybrid nanomaterials and a potential avenue toward new mechanistic insights in virus phenomena originating in many-body effects, which cannot be understood from examining the properties of molecular subunits alone. This approach complements the successful molecular biology perspective traditionally used in virology, and promises a deeper understanding of viruses and virus-like particles through an expanded methodological toolbox. Here we present protocols for forming a virus coat protein shell around functionalized inorganic nanoparticles.
以纳米颗粒为模板组装病毒外壳为混合纳米材料的生产提供了一种很有前景的方法，也为源自多体效应的病毒现象获得新的机理见解提供了一条潜在途径，仅通过研究分子亚基的特性无法理解这些现象。这种方法补充了病毒学中传统使用的成功的分子生物学观点，并有望通过扩展的方法工具箱更深入地理解病毒和病毒样颗粒。在这里，我们展示了在功能化无机纳米颗粒周围形成病毒衣壳蛋白外壳的方案。
