de Koning R W, Daemen M, Schutte B, Geertzen H G, Blijham G H, Bosman F T
Eur J Clin Invest. 1985 Apr;15(2):64-8. doi: 10.1111/j.1365-2362.1985.tb00146.x.
Detection of liver cell membrane autoantibodies is routinely performed by immunofluorescence testing of patient sera on rabbit hepatocyte suspensions. We have investigated the possible use of cells from the PLC/PRF/5 human hepatoma cell-line. These cells were employed as substrate in an immunofluorescence test which was compared with the conventional rabbit hepatocyte assay. We found a close correlation between the results obtained with these different substrates on visual reading. We furthermore compared visual reading of immunofluorescence preparations with flow-cytometrical analysis of immunostained cell suspensions. The results with these different methods were largely confirmatory. The PLC/PRF/5 cells are easily available and should therefore be regarded as a highly valuable new substrate for detection of liver cell membrane autoantibodies. Flow cytometry appears to be a technically simple and reliable method for quantitative analysis.
通过对患者血清在兔肝细胞悬液上进行免疫荧光检测来常规检测肝细胞膜自身抗体。我们研究了使用PLC/PRF/5人肝癌细胞系细胞的可能性。这些细胞被用作免疫荧光试验的底物，并与传统的兔肝细胞检测方法进行比较。我们发现在视觉读数上，使用这些不同底物获得的结果之间存在密切相关性。此外，我们将免疫荧光制剂的视觉读数与免疫染色细胞悬液的流式细胞术分析进行了比较。这些不同方法的结果在很大程度上是相互印证的。PLC/PRF/5细胞很容易获得，因此应被视为检测肝细胞膜自身抗体的一种极有价值的新底物。流式细胞术似乎是一种技术上简单且可靠的定量分析方法。
