Datta Sudeshna, Kanjilal Baishali, Sarkar Priyabrata
a Department of Polymer Science and technology , University of Calcutta , Calcutta , West Bengal , India.
b Institute of Material Sciences, University of Connecticut , Storrs , Connecticut , USA.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2018;53(12):1048-1055. doi: 10.1080/10934529.2018.1474574. Epub 2018 Jun 5.
In this paper a simple electrochemical sensing of dopamine by a new effective immobilization of tyrosinase (Tyr) enzyme on eggshell membrane (ESM) along with silver nanoparticles (AgNPs) is reported. The modified membrane was characterized by scanning electron microscope (SEM), energy dispersive spectroscopy (EDAX), X-Ray diffraction (XRD). A simple solution based approach was used to prepare AgNPs on biomembrane followed by glutaraldehyde activation to immobilize Tyr on the nanoparticles decorated ESM. The direct electrochemistry of DA oxidation was performed through cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Characterization of membrane was accomplished by electrochemical impedance spectroscopy (EIS). Prepared electrode showed very good stability, reproducibility, high selectivity, easy preparation and regeneration of electrode. The proposed sensor exhibited low detection limits 1.7ngL with wide linear range 10-1000 ngL, excellent sensitivity (14.28µA µgLcm) with good storage and operational stabilities. The accurate measurement of dopamine in blood serum and good recoveries in spiked serum samples ensured great potential for medical diagnostics.
本文报道了一种通过将酪氨酸酶（Tyr）有效固定在蛋壳膜（ESM）上并结合银纳米颗粒（AgNPs）来实现多巴胺的简单电化学传感方法。通过扫描电子显微镜（SEM）、能量色散光谱（EDAX）、X射线衍射（XRD）对修饰后的膜进行了表征。采用一种基于简单溶液的方法在生物膜上制备AgNPs，随后通过戊二醛活化将Tyr固定在纳米颗粒修饰的ESM上。通过循环伏安法（CV）和差分脉冲伏安法（DPV）对多巴胺氧化的直接电化学进行了研究。通过电化学阻抗谱（EIS）对膜进行了表征。制备的电极表现出非常好的稳定性、重现性、高选择性、易于制备和电极再生。所提出的传感器具有低检测限1.7ng/L、宽线性范围10 - 1000ng/L、优异的灵敏度（14.28µA/µg/L/cm）以及良好的储存和操作稳定性。血清中多巴胺的准确测量以及加标血清样品中的良好回收率确保了其在医学诊断方面的巨大潜力。
