Glycomyces sediminimaris sp. nov., a new species of actinobacteria isolated from marine sediment.

A novel Glycomyces strain, designated as MH2460, was isolated from marine sediment collected from 12 m depth in Rostami seaport, Bushehr Province in Iran. On International Streptomyces Project 2 medium it produced branching substrate hyphae that developed into a large number of irregularly shaped spores in 8 days. It showed optimal growth at 25-35 °C, pH 6.0-8.0 and in salinity between 2.5-5 % (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched descriptions for members of the genus Glycomyces. Whole-cell hydrolysates of strain MH2460 contained meso-diaminopimelic acids along with glucose, ribose and small traces of xylose and galactose. The phospholipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides as well as two unidentified phosphoglycolipids, one unidentified phospholipid and an unidentified aminolipid. The predominant menaquinones were MK-11(H4) and MK-10(H4). The fatty-acid pattern was mainly composed of anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The strain belongs to the genus Glycomyces based on 16S rRNA gene sequence with the highest pairwise sequence identity (98.3 %) with Glycomyces phytohabitans KLBMP 1483. The DNA-DNA hybridization value showed 53.9±2.7 % identity when MH2460 was compared to this reference strain. The G+C content of the DNA was 70.2 mol%. Based on phenotypic, biochemical, chemotaxonomic and genotypic features, strain MH2460 (DSM 103727=UTMC 2460=NCCB 100631) is considered to represent a novel species of the genus Glycomyces, for which the name Glycomyces sediminimaris is proposed.