College of Life Science/Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar 843300, PR China.
Int J Syst Evol Microbiol. 2018 Sep;68(9):2722-2726. doi: 10.1099/ijsem.0.002842. Epub 2018 Jul 9.
A novel actinomycete, strain TRM 41368, was isolated from a silt sample from Xiaoerkule lake in Xinjiang province, China, and was examined using a polyphasic approach. Strain TRM 41368 was aerobic, Gram-stain-positive, with an optimum NaCl concentration for growth of 5 % (w/v), and an optimum temperature for growth of 35-37 °C. On the basis of 16S rRNA gene sequence analysis, strain TRM 41368 was most closely related to Glycomycesfuscus TRM 49117 (98.46 % similarity). However, it had a relatively low DNA-DNA relatedness value with G. fuscus TRM 49117 (ANI=70.59 %). The organism had chemical and morphological features typical of the genus Glycomyces. The cell wall of TRM 41368 contained meso-diaminopimelic acid; xylose, ribose and glucose were the major whole-cell sugars. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositolmannosides. The predominant menaquinone was MK-9(H6). The major fatty acids were C18 : 1ω9c, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0. The G+C content of the DNA was 69.9 mol%. On the basis of the polyphasic evidence, strain TRM 41368 should be designated as a novel species of the genus Glycomyces, for which the name Glycomyces xiaoerkulensis sp. nov. is proposed. The type strain is TRM 41368 (=CCTCC AA 2017005=KCTC 39932).
一株新的放线菌，菌株 TRM 41368，从中国新疆小萼尔库里湖的淤泥样品中分离出来，并通过多相方法进行了研究。菌株 TRM 41368 为需氧、革兰氏阳性，最适生长的 NaCl 浓度为 5%（w/v），最适生长温度为 35-37°C。根据 16S rRNA 基因序列分析，菌株 TRM 41368 与 Glycomycesfuscus TRM 49117 最为密切相关（相似度为 98.46%）。然而，它与 G. fuscus TRM 49117 的 DNA-DNA 相关性值相对较低（ANI=70.59%）。该菌具有典型的糖单胞菌属的化学和形态特征。TRM 41368 的细胞壁含有 meso-二氨基庚二酸；主要的全细胞糖为木糖、核糖和葡萄糖。诊断性极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要的menaquinone 为 MK-9(H6)。主要脂肪酸为 C18:1ω9c、C16:0、iso-C16:0、anteiso-C17:0 和 anteiso-C15:0。DNA 的 G+C 含量为 69.9mol%。基于多相证据，菌株 TRM 41368 应被指定为糖单胞菌属的一个新种，命名为 Glycomyces xiaoerkulensis sp. nov.。模式菌株为 TRM 41368（=CCTCC AA 2017005=KCTC 39932）。
