Yagura T, Nagata I, Kuma K, Uchino H
J Clin Endocrinol Metab. 1985 Jun;60(6):1180-6. doi: 10.1210/jcem-60-6-1180.
Phosphodiesterase (PDE) and calmodulin (CaM) activities were studied in soluble fractions of normal and Graves' thyroid tissue. Normal and Graves' thyroid tissues were obtained at thyroid surgery. PDE activities were assayed with cAMP and cGMP as substrates. CaM activity was measured as the ability to activate bovine thyroid CaM-dependent PDE. cAMP and cGMP PDE activities were increased 1.5- and 2.2-fold above normal in Graves' thyroid, respectively. The major cause of the increase in enzyme activities was their higher Ca+2 dependence. CaM activity also was 1.6-fold increased in Graves' thyroid, although it probably does not contribute to the increase in the Ca+2 dependence of PDE activities because of its relative sufficiency vs. PDE in normal thyroid. Three forms of cAMP and cGMP PDE activities were eluted from Sephadex G-200 columns, with mol wt of 280,000, 140,000, and 80,000-100,000, respectively. The first peak had little or no CaM dependence, the second peak had moderate or dominant CaM dependence, and the third peak revealed weak or dominant CaM dependence. The increase in the CaM-dependent form of the third peak of PDE activity in Graves' thyroid tissue may explain the increase in Ca+2 dependence of PDE activities.
在正常和格雷夫斯病甲状腺组织的可溶性部分研究了磷酸二酯酶（PDE）和钙调蛋白（CaM）的活性。正常和格雷夫斯病甲状腺组织取自甲状腺手术。以环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）为底物测定PDE活性。通过激活牛甲状腺CaM依赖性PDE的能力来测量CaM活性。在格雷夫斯病甲状腺中，cAMP和cGMP PDE活性分别比正常水平增加了1.5倍和2.2倍。酶活性增加的主要原因是它们对Ca2+的依赖性更高。格雷夫斯病甲状腺中的CaM活性也增加了1.6倍，尽管由于其在正常甲状腺中相对于PDE相对充足，它可能对PDE活性的Ca2+依赖性增加没有贡献。从葡聚糖G - 200柱上洗脱得到三种形式的cAMP和cGMP PDE活性，分子量分别为280,000、140,000和80,000 - 100,000。第一个峰对CaM的依赖性很小或没有，第二个峰具有中等或主要的CaM依赖性，第三个峰显示出弱或主要的CaM依赖性。格雷夫斯病甲状腺组织中PDE活性第三个峰的CaM依赖性形式的增加可能解释了PDE活性Ca2+依赖性的增加。
