University of Arkansas, Department of Biomedical Engineering, Fayetteville, Arkansas, United States.
J Biomed Opt. 2018 Jun;23(6):1-6. doi: 10.1117/1.JBO.23.6.067001.
Tumor hypoxia is a critical indicator of poor clinical outcome in patients with cancers of the breast, cervix, and oral cavity. The ability to noninvasively and reliably monitor tumor oxygenation both prior to and during therapy can aid in identifying poor treatment response earlier than is currently possible and lead to effective changes in treatment regimen. Diffuse reflectance spectroscopy (DRS) has been used in several studies to measure tissue scattering, total hemoglobin content (THb), and vascular oxygenation (sO2) in tissue. In this study, we validate in vivo DRS-based measurements of vascular oxygenation using immunohistochemical staining of tumor hypoxia using pimonidazole, an established hypoxia marker. Using tumor xenografts grown from two different head and neck cell lines-UM-SCC-22B and UM-SCC-47-we demonstrate statistically significant negative correlations between tumor hypoxic fraction (HF) and THb (r = - 0.45; p = 0.04) and sO2 (r = - 0.50; p = 0.02). In addition, we also found a statistically significant positive correlation between HF and mean reduced scattering coefficient (r = 0.60; p = 0.005). Our results demonstrate that DRS-based measures of sO2 can provide reliable indirect measurements of tumor hypoxia that can be of significant utility in preclinical and clinical studies.
肿瘤缺氧是乳腺癌、宫颈癌和口腔癌患者临床预后不良的一个关键指标。在治疗前和治疗过程中能够非侵入性且可靠地监测肿瘤氧合情况，有助于更早地识别治疗反应不良，并导致治疗方案的有效改变。漫反射光谱（DRS）已在多项研究中用于测量组织散射、总血红蛋白含量（THb）和组织中的血管氧合（sO2）。在这项研究中，我们使用缺氧标志物 pimonidazole 对肿瘤缺氧进行免疫组织化学染色，验证了基于 DRS 的血管氧合的体内测量。我们使用来自两种不同的头颈部细胞系-UM-SCC-22B 和 UM-SCC-47-生长的肿瘤异种移植物，证明了肿瘤缺氧分数（HF）与 THb（r = -0.45；p = 0.04）和 sO2（r = -0.50；p = 0.02）之间存在显著的负相关。此外，我们还发现 HF 与平均散射系数（r = 0.60；p = 0.005）之间存在显著的正相关。我们的结果表明，基于 DRS 的 sO2 测量可以提供肿瘤缺氧的可靠间接测量，这在临床前和临床研究中具有重要的应用价值。
