Abdel-Salam Ibrahim M, Ashmawy Abeer M, Hilal Amany M, Eldahshan Omayma A, Ashour Mohamed
Department of Cancer Biology National Cancer Institute, Cairo University, Kasr Al Eini Street, Fom El Khalig, 11796-, Cairo, Egypt.
Department of Medical Oncology, National Cancer Institute, Cairo University, Cairo, Egypt.
Chem Biodivers. 2018 Aug;15(8):e1800045. doi: 10.1002/cbdv.201800045. Epub 2018 Jul 21.
Breast cancer constitutes the second most prevalent cancer in Egypt, the problem needs more trends in treatment and treatment development either by regimen modification or introducing new drugs, and the main objective of this study is to screen the effects of the aqueous ethanol herbal extract of Luffa cylindrica leaves on different types of breast cancer cell lines representing different molecular subtypes of the disease. The major active constituents of the extract were tentatively identified by LC/MS which revealed the presence of phenolic compound derivatives and saponin that may be responsible in part for the activity of the extract. The emphasis was laid on the main apoptotic pathways as well as the extract effect on the normal cell line. Results of phytochemical investigation, cell cycle analysis, and molecular analysis of apoptotic and proliferative markers have shown effective anticancer activity against MCF-7, BT-474, and MDA-MB-231 cell lines which represent three subtypes of breast cancer, luminal A, luminal B, and triple negative, respectively. On the other hand, the effects on normal lung fibroblast cell line are less prominent at the dose used for treating breast cancer cell lines.
乳腺癌是埃及第二大常见癌症，该问题需要在治疗和治疗发展方面有更多趋势，无论是通过方案修改还是引入新药，本研究的主要目的是筛选丝瓜叶水乙醇草药提取物对代表该疾病不同分子亚型的不同类型乳腺癌细胞系的影响。通过液相色谱/质谱法初步鉴定了提取物的主要活性成分，结果显示存在酚类化合物衍生物和皂苷，它们可能部分负责提取物的活性。重点放在主要的凋亡途径以及提取物对正常细胞系的影响上。植物化学研究、细胞周期分析以及凋亡和增殖标志物的分子分析结果表明，该提取物对代表乳腺癌三种亚型（分别为管腔A型、管腔B型和三阴性）的MCF-7、BT-474和MDA-MB-231细胞系具有有效的抗癌活性。另一方面，在用于治疗乳腺癌细胞系的剂量下，对正常肺成纤维细胞系的影响不太明显。
