Giberson Andrea N, Saha Bratati, Campbell Kalisa, Christou Carin, Poulin Kathy L, Parks Robin J
a Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
b Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
Biochem Cell Biol. 2018 Dec;96(6):797-807. doi: 10.1139/bcb-2018-0117. Epub 2018 Jun 6.
Adenovirus (Ad) DNA undergoes dynamic changes in protein association as the virus progresses through its replicative cycle. Within the virion, the Ad DNA associates primarily with the virus-encoded, protamine-like protein VII. During the early phase of infection (∼6 h), the viral DNA showed declining association with VII, suggesting that VII was removed from at least some regions of the viral DNA. Within 6 h, the viral DNA was wrapped into a repeating nucleosome-like array containing the histone variant H3.3. Transcription elongation was not required to strip VII from the viral DNA or for deposition of H3.3. H3.1 did not associate with the viral DNA at any point during infection. During the late phase of infection (i.e., active DNA replication ∼12-24 h), association with H3 was dramatically reduced and the repeating nucleosome-like pattern was no longer evident. Thus, we have uncovered some of the changes in nucleoprotein structure that occur during lytic Ad infection.
随着腺病毒（Ad）在其复制周期中进展，腺病毒DNA在蛋白质结合方面会发生动态变化。在病毒粒子内，腺病毒DNA主要与病毒编码的、鱼精蛋白样蛋白VII结合。在感染的早期阶段（约6小时），病毒DNA与VII的结合减少，这表明VII从病毒DNA的至少一些区域被移除。在6小时内，病毒DNA被包裹成含有组蛋白变体H3.3的重复核小体样阵列。转录延伸并非从病毒DNA上剥离VII或沉积H3.3所必需的。在感染的任何阶段，H3.1都不与病毒DNA结合。在感染的后期阶段（即活跃的DNA复制约12 - 24小时），与H3的结合显著减少，重复的核小体样模式不再明显。因此，我们揭示了在腺病毒裂解感染期间发生的一些核蛋白结构变化。
