Ehrlich M G
J Orthop Res. 1985;3(2):170-84. doi: 10.1002/jor.1100030206.
In osteoarthritis, despite increased matrix synthesis, there is a reduction of both major matrix components, proteoglycan and collagen. This study suggests that this is the result of enhanced degradative activity intrinsic to the cartilage. Because osteoarthritis is a focal disease, histologic controls were used to measure the severity in different areas of the cartilage and in different specimens. Neutral proteoglycanase and collagenase were both described in human cartilage, and their levels matched the severity of the disease as did acid phosphatase, a marker of lysosomal enzymes. Articular cartilage collagenase has an inhibitor in the cartilage and has negligible activity in normal cartilage. This was found not to be a lysosomal enzyme. A model of osteoarthritis was studied and found to have the same biochemical pattern as human disease. Using this method, inhibitors of degradative enzymes were used as a treatment. The chelator of metallic cations EDTA was found to have a significant effect on reduction of degradative enzyme activity and altered the arthritic process.
在骨关节炎中，尽管基质合成增加，但蛋白聚糖和胶原蛋白这两种主要基质成分均减少。本研究表明，这是软骨固有降解活性增强的结果。由于骨关节炎是一种局灶性疾病，因此采用组织学对照来测量软骨不同区域和不同标本中的严重程度。中性蛋白聚糖酶和胶原酶在人体软骨中均有描述，它们的水平与疾病严重程度相符，溶酶体酶标志物酸性磷酸酶也是如此。关节软骨胶原酶在软骨中有抑制剂，在正常软骨中的活性可忽略不计。已发现该抑制剂不是溶酶体酶。对骨关节炎模型进行了研究，发现其具有与人类疾病相同的生化模式。使用该方法，将降解酶抑制剂用作治疗手段。发现金属阳离子螯合剂乙二胺四乙酸（EDTA）对降低降解酶活性有显著作用，并改变了关节炎进程。
