Research Group on Human Health and Environment, Feevale University, Brazil.
Research Group on Human Health and Environment, Feevale University, Brazil.
Chemosphere. 2018 Feb;193:1058-1062. doi: 10.1016/j.chemosphere.2017.11.119. Epub 2017 Nov 22.
Workers involved in urban solid waste collection may be exposed to various environmental contaminants, including chemical pollutants, which might be mutagenic and increase the risk of diseases such as cancer. Evaluation of DNA damage in workers in this field are still scarce. This study aims to evaluate mutagenic and cytotoxic effects in workers involved in the collection and segregation of urban solid waste generated in southern Brazil. Municipal solid waste collectors were recruited in two municipalities of the state of Rio Grande do Sul. The control group was composed of workers of the education and commerce areas, with no exposure to known genotoxic agents. Slides of exfoliated buccal cells were analyzed to estimate the frequency of micronuclei (MN) as well as other nuclear abnormalities, such as broken-egg/bud, binucleation and karyorrhexis. The analyses of 44 workers and 45 control subjects have shown that the frequencies of MN, binucleated cells and karyorrhexis in the exposed workers were significantly higher than in the control group. In the exposed group, frequencies of MN and binucleated cells showed a significant positive correlation. The other cytogenetic parameters were not correlated among each other or with age and exposure time. These results indicate that the workers involved in urban solid waste collection are exposed to mutagenic and cytotoxic agents.
从事城市固体废物收集的工人可能会接触到各种环境污染物，包括化学污染物，这些污染物可能具有致突变性，并增加癌症等疾病的风险。对于从事这项工作的工人的 DNA 损伤评估仍然很少。本研究旨在评估巴西南部产生的城市固体废物收集和分类工作者的致突变和细胞毒性作用。在南里奥格兰德州的两个城市招募了城市固体废物收集者。对照组由教育和商业领域的工人组成，他们没有接触过已知的遗传毒性剂。分析脱落的口腔细胞载玻片以估计微核（MN）的频率以及其他核异常，如破碎的卵/芽、双核和核溶解。对 44 名工人和 45 名对照者的分析表明，暴露组的 MN、双核细胞和核溶解的频率明显高于对照组。在暴露组中，MN 和双核细胞的频率呈显著正相关。其他细胞遗传学参数彼此之间或与年龄和暴露时间均无相关性。这些结果表明，从事城市固体废物收集的工人接触到致突变和细胞毒性剂。
