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三氯乙酸沉淀法和乙醇/盐酸单步纯化评估：关于人血清白蛋白、核糖核酸酶、溶菌酶的一维凝胶电泳、考马斯亮蓝法、荧光分光光度法和拉曼光谱数据——Mascots和Skyline数据。

TCA precipitation and ethanol/HCl single-step purification evaluation: One-dimensional gel electrophoresis, bradford assays, spectrofluorometry and Raman spectroscopy data on HSA, Rnase, lysozyme - Mascots and Skyline data.

作者信息

Eddhif Balkis, Guignard Nadia, Batonneau Yann, Clarhaut Jonathan, Papot Sébastien, Geffroy-Rodier Claude, Poinot Pauline

机构信息

Institut de Chimie des Milieux et des Matériaux de Poitiers (IC2MP), Université de Poitiers, CNRS, Equipe Eaux, Biomarqueurs, Contaminants Organiques, Milieux, (E-BiCOM), 4 rue Michel Brunet, TSA 51106, F-86073 Poitiers, France.

Institut de Chimie des Milieux et des Matériaux de Poitiers (IC2MP), Université de Poitiers, CNRS, Equipe Site Actif au Matériau Catalytique (SAMCat), 4 rue Michel Brunet, TSA 51106, F-86073 Poitiers, France.

出版信息

Data Brief. 2018 Feb 5;17:938-953. doi: 10.1016/j.dib.2018.01.095. eCollection 2018 Apr.

DOI:10.1016/j.dib.2018.01.095
PMID:29876449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5988388/
Abstract

The data presented here are related to the research paper entitled "Study of a Novel Agent for TCA Precipitated Proteins Washing - Comprehensive Insights into the Role of Ethanol/HCl on Molten Globule State by Multi-Spectroscopic Analyses" (Eddhif et al., submitted for publication) [1]. The suitability of ethanol/HCl for the washing of TCA-precipitated proteins was first investigated on standard solution of HSA, cellulase, ribonuclease and lysozyme. Recoveries were assessed by one-dimensional gel electrophoresis, Bradford assays and UPLC-HRMS. The mechanistic that triggers protein conformational changes at each purification stage was then investigated by Raman spectroscopy and spectrofluorometry. Finally, the efficiency of the method was evaluated on three different complex samples (mouse liver, river biofilm, loamy soil surface). Proteins profiling was assessed by gel electrophoresis and by UPLC-HRMS.

摘要

此处呈现的数据与题为《一种用于三氯乙酸沉淀蛋白洗涤的新型试剂的研究——通过多光谱分析深入了解乙醇/盐酸对熔球态的作用》的研究论文相关（Eddhif等人，已提交发表）[1]。首先在人血清白蛋白、纤维素酶、核糖核酸酶和溶菌酶的标准溶液上研究了乙醇/盐酸用于洗涤三氯乙酸沉淀蛋白的适用性。通过一维凝胶电泳、Bradford测定法和超高效液相色谱-高分辨质谱法评估回收率。然后通过拉曼光谱和荧光光谱法研究在每个纯化阶段触发蛋白质构象变化的机制。最后，在三种不同的复杂样品（小鼠肝脏、河流生物膜、壤土表面）上评估该方法的效率。通过凝胶电泳和超高效液相色谱-高分辨质谱法评估蛋白质谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/5a9603cc06af/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/d1fa24227e9b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/d8fedd5ab1fc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/51be89313103/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/ece30870fffe/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/681fd351d614/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/fb9301680b89/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/724ba4262b5d/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/75d48e067464/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/5214d03bbbf9/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/096b260a9177/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/5a9603cc06af/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/d1fa24227e9b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/d8fedd5ab1fc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/51be89313103/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/ece30870fffe/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/681fd351d614/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/fb9301680b89/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/724ba4262b5d/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/75d48e067464/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/5214d03bbbf9/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/096b260a9177/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8c/5988388/5a9603cc06af/gr11.jpg

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