Kovar H, Wintersberger E
Mol Gen Genet. 1985;199(1):146-51. doi: 10.1007/BF00327524.
We have produced nonviable deletion mutants of polyoma virus in order to study homologous recombination after DNA transfection into mouse cells. The frequency of recombination was determined by the formation of infectious virus. It was dependent on the amount of DNA transfected and the size of the region of homology between the mutations. Recombination frequencies were highest when both mutated genomes were transfected in closed circular form rather than after linearization of one or both of the recombination partners. The system described may be useful for a more detailed analysis of physiological and genetic conditions influencing the frequency of homologous recombination in mouse cells as well as to study enzymes involved and intermediates produced in this process.
我们制备了多瘤病毒的无活力缺失突变体，以便研究DNA转染小鼠细胞后的同源重组。重组频率通过感染性病毒的形成来确定。它取决于转染的DNA量以及突变之间同源区域的大小。当两个突变基因组以闭环形式转染时，重组频率最高，而不是在一个或两个重组伙伴线性化之后。所描述的系统可能有助于更详细地分析影响小鼠细胞中同源重组频率的生理和遗传条件，以及研究此过程中涉及的酶和产生的中间体。
