Intarakumhaeng R, Shi Z, Wanasathop A, Stella Q C, Wei K S, Styczynski P B, Li C, Smith E D, Li S K
Division of Pharmaceutical Sciences, James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, OH, 45267, USA.
Procter& Gamble Co. (P&G), Mason, OH, 45050, USA.
Int J Cosmet Sci. 2018 Aug;40(4):367-376. doi: 10.1111/ics.12469. Epub 2018 Aug 15.
Petrolatum and soybean oil are common ingredients incorporated in topical skin formulations for skin protection and moisturization. However, the stratum corneum (SC) penetration kinetics of these two cosmetic ingredients has not been systematically studied. Glyceryl monooleate (GlyMOle) has been shown to enhance skin penetration of various compounds. It was hypothesized that GlyMOle could enhance skin penetration of petrolatum and soybean oil. This study aimed to examine the in vitro skin penetration of petrolatum and soybean oil in the presence or absence of GlyMOle.
Skin permeation experiments were conducted using the in vitro Franz diffusion cell model with split-thickness human skin and human epidermal membrane (HEM). The effect of permeant dose and the kinetics of permeant penetration were examined with and without GlyMOle in vitro.
Petrolatum and soybean oil were found to permeate across HEM, and no effect of GlyMOle on skin permeation into the receptor chamber was observed. GlyMOle enhanced the penetration of petrolatum into the split-thickness skin at 50 μg dose (petrolatum:GlyMOle, 49 : 1, w/w). However, no effect of GlyMOle on petrolatum penetration was observed at 200 μg dose (of the same petrolatum:GlyMOle ratio), indicating a dose-dependent effect. GlyMOle at the level used in the study did not enhance the penetration of soybean oil with 50 and 200 μg doses at any timepoints.
GlyMOle was a skin penetration enhancer for petrolatum under the in vitro conditions identified in this study.
凡士林和大豆油是用于皮肤保护和保湿的局部皮肤制剂中常见的成分。然而，这两种化妆品成分在角质层（SC）中的渗透动力学尚未得到系统研究。单油酸甘油酯（GlyMOle）已被证明可增强多种化合物的皮肤渗透性。据推测，GlyMOle可增强凡士林和大豆油的皮肤渗透性。本研究旨在考察在有或没有GlyMOle存在的情况下凡士林和大豆油的体外皮肤渗透性。
使用具有人断层皮肤和人表皮膜（HEM）的体外Franz扩散池模型进行皮肤渗透实验。在体外考察了渗透剂剂量和渗透剂渗透动力学在有或没有GlyMOle情况下的情况。
发现凡士林和大豆油可透过HEM，未观察到GlyMOle对皮肤向接受室渗透有影响。在50μg剂量（凡士林：GlyMOle，49:1，w/w）时，GlyMOle增强了凡士林向断层皮肤的渗透。然而，在200μg剂量（相同的凡士林：GlyMOle比例）时，未观察到GlyMOle对凡士林渗透有影响，表明存在剂量依赖性效应。在本研究中使用的GlyMOle水平在任何时间点均未增强50μg和200μg剂量大豆油的渗透。
在本研究确定的体外条件下，GlyMOle是凡士林的皮肤渗透促进剂。
